細胞材料開発室サイト
検索画面


戻る 戻る
RCB0007 : HeLa  update : 2018/07/06
CommentJust like ATCC CCL 2, HeLa
特性(日) 培養細胞の中で最もよく利用されるヒト子宮頸部癌細胞である。多数の亜株があるが、これはGeyのオリジナル株由来である。
寄託者特性(英)
寄託者特性(日)
使用条件a
使用条件(英)Basically, there is no restriction regarding academic use.
使用条件(日)学術研究における利用につきましては原則として使用制限はありません。
提供申込書類 依頼書C-0001.pdf   同意書(非営利)C-0003.pdf   同意書(営利)C-0003p.pdf  
提供同意書は、使用機関及び使用内容に応じて二種類あります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は別途ご相談(cellbank.brc@riken.jp)を承ります。
細胞基本情報 寄託者 Soeda, Eiichi
樹立者 Gey, G. O. et al.
寄託日 1986
動物種 human < Mammals
属名 Homo
種名 sapiens
性別 Female
31 years
採取組織 cervix
病歴 cervical carcinoma
細胞分類 cancer
初代培養日 1952
細胞寿命 infinite
細胞形態 epithelial-like
細胞培養情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 10% CS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 8 split
継代または培地交換頻度 Subculture : 1-2 times/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
個体識別検査 OK
ウィルス(HIV) (-)
アイソザイム検査 LD, NP
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 0件
Reference(日) 0件
利用者成果(英) 52件
利用者成果(日) 0件

トップへページトップへ
Reference(英)

トップへページトップへ
Reference(日)

トップへページトップへ
利用者成果(英)
Akita, Hidetaka, Tanimoto, Mitsuhide, Masuda, Tomoya, Kogure, Kentaro, Hama, Susumu, Ninomiya, Keiko, Futaki, Shiroh, Harashima, Hideyoshi  Evaluation of the nuclear delivery and intra-nuclear transcription of plasmid DNA condensed with micro (mu) and NLS-micro by cytoplasmic and nuclear microinjection: a comparative study with poly-L-lysine.  J Gene Med  2006  8:198-206 
Bonneau B, Ando H, Kawaai K, Hirose M, Takahashi-Iwanaga H, Mikoshiba K.  IRBIT controls apoptosis by interacting with the Bcl-2 homolog, Bcl2l10, and by promoting ER-mitochondria contact.  Elife  2016  5:  PubMed ID: 27995898
Ueyama T, Ninoyu Y, Nishio SY, Miyoshi T, Torii H, Nishimura K, Sugahara K, Sakata H, Thumkeo D, Sakaguchi H, Watanabe N, Usami SI, Saito N, Kitajiri SI.  Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss.  EMBO Mol Med  2016  8:1310-1324  PubMed ID: 27707755
Kanda S, Yanagitani K, Yokota Y, Esaki Y, Kohno K.  Autonomous translational pausing is required for XBP1u mRNA recruitment to the ER via the SRP pathway.  Proc. Natl. Acad. Sci. U.S.A.  2016  113:E5886-E5895  PubMed ID: 27651490
Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, Kataoka K, Saito T, Chung UI, Ohba S.  Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment.  Sci Rep  2016  6:18743  PubMed ID: 26728350
Bannai H, Niwa F, Sherwood MW, Shrivastava AN, Arizono M, Miyamoto A, Sugiura K, Levi S, Triller A, Mikoshiba K.  Bidirectional Control of Synaptic GABAAR Clustering by Glutamate and Calcium.  Cell Rep  2015  13:2768-80  PubMed ID: 26711343
Abe N, Matsumoto K, Nishihara M, Nakano Y, Shibata A, Maruyama H, Shuto S, Matsuda A, Yoshida M, Ito Y, Abe H.  Rolling Circle Translation of Circular RNA in Living Human Cells.  Sci Rep  2015  5:16435  PubMed ID: 26553571
Kobayashi S, Koujin T, Kojidani T, Osakada H, Mori C, Hiraoka Y, Haraguchi T.  BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy.  Proc. Natl. Acad. Sci. U.S.A.  2015  112:7027-32  PubMed ID: 25991860
Kondo T, Isoda R, Uchimura T, Sugiyama M, Hamao K, Hosoya H.  Diphosphorylated but not monophosphorylated myosin II regulatory light chain localizes to the midzone without its heavy chain during cytokinesis.  Biochem. Biophys. Res. Commun.  2011  417:686-91  PubMed ID: 22166199
Satow R, Shitashige M, Jigami T, Fukami K, Honda K, Kitabayashi I, Yamada T.  β-catenin inhibits promyelocytic leukemia protein tumor suppressor function in colorectal cancer cells.  Gastroenterology  2011  142:572-81  PubMed ID: 22155184
Ochiai K, Yoshikawa Y, Yoshimatsu K, Oonuma T, Tomioka Y, Takeda E, Arikawa J, Mominoki K, Omi T, Hashizume K, Morimatsu M.  Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51.  FEBS Lett.  2011  585:1771-7  PubMed ID: 21601571
Okano K, Yu D, Matsui A, Maezawa Y, Hosokawa Y, Kira A, Matsubara M, Liau I, Tsubokawa H, Masuhara H.  Induction of cell-cell connections by using in situ laser lithography on a perfluoroalkyl-coated cultivation platform.  Chembiochem  2011  12:795-801  PubMed ID: 21341350
Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550
Watanabe S, Wakasugi K.  Module M1 of zebrafish neuroglobin acts as a structural and functional protein building block for a cell-membrane-penetrating activity.  PLoS ONE  2011  6:e16808  PubMed ID: 21304818
Kondo T, Hamao K, Kamijo K, Kimura H, Morita M, Takahashi M, Hosoya H.  Enhancement of myosin II/actin turnover at the contractile ring induces slower furrowing in dividing HeLa cells.  Biochem. J.  2011  435:569-76  PubMed ID: 21231914
Kaneko S, Nakayama H, Yoshino Y, Fushimi D, Yamaguchi K, Horiike Y, Nakanishi J.  Photocontrol of cell adhesion on amino-bearing surfaces by reversible conjugation of poly(ethylene glycol) via a photocleavable linker.  Phys Chem Chem Phys  2011  13:4051-9  PubMed ID: 21225032
Konno Y, Aoki M, Takagishi M, Sakai N, Koike M, Wakamatsu K, Hosoi S.  Enhancement of antibody production by the addition of Coenzyme-Q(10).  Cytotechnology  2011  63:163-70  PubMed ID: 21197574
Hakoda M, Wakizaka Y, Hirota Y.  Separation of viable and nonviable animal cell using dielectrophoretic filter.  Biotechnol. Prog.  2010  26:1061-7  PubMed ID: 20205163
Li F, Awale S, Tezuka Y, Kadota S.  Cytotoxic constituents of propolis from Myanmar and their structure-activity relationship.  Biol. Pharm. Bull.  2009  32:2075-8  PubMed ID: 19952433
Awale S, Miyamoto T, Linn TZ, Li F, Win NN, Tezuka Y, Esumi H, Kadota S.  Cytotoxic constituents of Soymida febrifuga from Myanmar.  J. Nat. Prod.  2009  72:1631-6  PubMed ID: 19689125
Kimura T, Hashimoto I, Nishikawa M, Yamada H.  Nucleocytoplasmic transport of luciferase gene mRNA requires CRM1/Exportin1 and RanGTPase.  Med Mol Morphol  2009  42:70-81  PubMed ID: 19536614
Asano S, Hamao K, Hosoya H.  Direct evidence for roles of phosphorylated regulatory light chain of myosin II in furrow ingression during cytokinesis in HeLa cells.  Genes Cells  2009  14:555-68  PubMed ID: 19371382
Hamao K, Morita M, Hosoya H.  New function of the proline rich domain in dynamin-2 to negatively regulate its interaction with microtubules in mammalian cells.  Exp. Cell Res.  2009  315:1336-45  PubMed ID: 19331814
Yamamoto N, Takemori Y, Sakurai M, Sugiyama K, Sakurai H.  Differential recognition of heat shock elements by members of the heat shock transcription factor family.  FEBS J.  2009  276:1962-74  PubMed ID: 19250318
Koichi Baba, Hitoshi Kasai, Akito Masuhara, Hidetoshi Oikawa, and Hachiro Nakanishi  Organic Solvent-Free Fluorescence Confocal Imaging of Living Cells Using Pure Nanocrystal Forms of Fluorescent Dyes  Jpn J Appl Phys.  2009  48:117002-1-117002-4 
Watanabe S, Wakasugi K.  Zebrafish neuroglobin is a cell-membrane-penetrating globin.  Biochemistry  2008  47:5266-70  PubMed ID: 18416560
Wakasugi K.  Human tryptophanyl-tRNA synthetase binds with heme to enhance its aminoacylation activity.  Biochemistry  2007  46:11291-8  PubMed ID: 17877375
Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.  Whole-mount immunoelectron tomography of chromosomes and cells.  Methods Mol. Biol.  2007  369:387-405  PubMed ID: 17656761
Nakayama T, Nomura N, Matsumura M.  The effect of copper concentration on the virulence of pathogenic Vibrio harveyi.  J. Appl. Microbiol.  2007  102:1300-6  PubMed ID: 17448165
Sasaki Y, Matsui K, Aoyama Y, Kikuchi J.  Cerasome as an infusible and cell-friendly gene carrier: synthesis of cerasome-forming lipids and transfection using cerasome.  Nat Protoc  2007  1:1227-34  PubMed ID: 17406406
Tanaka T, Takahashi M, Kusano E, Okamoto H.  Development and evaluation of an efficient cell-culture system for Hepatitis E virus.  J. Gen. Virol.  2007  88:903-11  PubMed ID: 17325363
Miyauchi K, Yamamoto Y, Kosaka T, Hosoya H.  Myosin II activity is not essential for recruitment of myosin II to the furrow in dividing HeLa cells.  Biochem. Biophys. Res. Commun.  2006  350:543-8  PubMed ID: 17022938
Horiuchi S, Aoyama Y.  Systematic lactose-functionalization of amphiphilic octaamine macrocycle as a gene carrier. Optimization of the charge, size, toxicity, and receptor factors for hepatocyte targeting.  J Control Release  2006  116:107-14  PubMed ID: 16905218
Nakayama T, Nomura N, Matsumura M.  Study on the relationship of protease production and luminescence in Vibrio harveyi.  J. Appl. Microbiol.  2006  101:200-5  PubMed ID: 16834607
Nakayama T, Ito E, Nomura N, Nomura N, Matsumura M.  Comparison of Vibrio harveyi strains isolated from shrimp farms and from culture collection in terms of toxicity and antibiotic resistance.  FEMS Microbiol. Lett.  2006  258:194-9  PubMed ID: 16640572
Matsui K, Horiuchi S, Sando S, Sera T, Aoyama Y.  RNAi silencing of exogenous and endogenous reporter genes using a macrocyclic octaamine as a "compact" siRNA carrier. Studies on the nonsilenced residual activity.  Bioconjug. Chem.  2006  17:132-8  PubMed ID: 16417261
Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, Ito S, Kurosawa K, Yamaguchi K, Sugiura Y, Takizaki H, Yamashita Y, Katakura R, Sato I, Kawai M, Okada Y, Watanabe H, Kondoh G, Matsumoto S, Kishimoto A, Obata M, Matsumoto M, Fukuhara T, Motohashi H, Suematsu M, Komatsu M, Nakayama KI, Watanabe T, Soga T, Shima H, Maemondo M, Tanuma N.  PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.  Cancer Cell  2018  33:355-367.e7  PubMed ID: 29533781
Yoshida W, Saikyo H, Nakabayashi K, Yoshioka H, Bay DH, Iida K, Kawai T, Hata K, Ikebukuro K, Nagasawa K, Karube I.  Identification of G-quadruplex clusters by high-throughput sequencing of whole-genome amplified products with a G-quadruplex ligand.  Sci Rep  2018  8:3116  PubMed ID: 29449667
Hane M, Kitajima K, Sato C.  Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products.  Biochem. Biophys. Res. Commun.  2016  478:1123-9  PubMed ID: 27565727
Lothrop BB, Mulla MS.  Mode of existence and seasonality of midge larvae (Diptera: Chironomidae) in man-made lakes in the Coachella Valley, southern California.  J. Am. Mosq. Control Assoc.  1995  11:77-85  PubMed ID: 7616195
Niwa F, Sakuragi S, Kobayashi A, Takagi S, Oda Y, Bannai H, Mikoshiba K.  Dissection of local Ca(2+) signals inside cytosol by ER-targeted Ca(2+) indicator.  Biochem. Biophys. Res. Commun.  2016  479:67-73  PubMed ID: 27616195
Hozumi Y, Fujiwara H, Kaneko K, Fujii S, Topham MK, Watanabe M, Goto K.  Diacylglycerol kinase ε localizes to subsurface cisterns of cerebellar Purkinje cells.  Cell Tissue Res.  2017  368:441-458  PubMed ID: 28191598
Ono T, Sakamoto C, Nakao M, Saitoh N, Hirano T.  Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ.  Mol. Biol. Cell  2017  28:2875-2886  PubMed ID: 28835373
Goto M, Osada S, Imagawa M, Nishizuka M.  FAD104, a regulator of adipogenesis, is a novel suppressor of TGF-β-mediated EMT in cervical cancer cells.  Sci Rep  2017  7:16365  PubMed ID: 29180690
Baba Y, Shigemi Z, Hara N, Moriguchi M, Ikeda M, Watanabe T, Fujimuro M.  Arctigenin induces the apoptosis of primary effusion lymphoma cells under conditions of glucose deprivation.  Int. J. Oncol.  2018  52:505-517  PubMed ID: 29207179
Yamamura T, Nozu K, Miyoshi Y, Nakanishi K, Fujimura J, Horinouchi T, Minamikawa S, Mori N, Fujimaru R, Nakanishi K, Ninchoji T, Kaito H, Mariko TI, Morioka I, Matsuo M, Iijima K.  An in vitro splicing assay reveals the pathogenicity of a novel intronic variant in ATP6V0A4 for autosomal recessive distal renal tubular acidosis.  BMC Nephrol  2017  18:353  PubMed ID: 29202719
Tsunoyama TA, Watanabe Y, Goto J, Naito K, Kasai RS, Suzuki KGN, Fujiwara TK, Kusumi A.  Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function.  Nat. Chem. Biol.  2018  14:497-506  PubMed ID: 29610485
Morisaku T, Yui H.  Laser-induced surface deformation microscope for the study of the dynamic viscoelasticity of plasma membrane in a living cell.  Analyst  2018  143:2397-2404  PubMed ID: 29700531
  Biocompatible CdSe/ZnS quantum dot micelles for long-term cell imaging without alteration to the native structure of the blood plasma protein human serum albumin        PubMed ID:
  Development of a Cartridge-type Cell Culture Device Combined with Poly(N-isopropylacrylamide) Coated Substrates        PubMed ID:
  Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation        PubMed ID:
  Characterization of tilapia sialidase and its significance in bacterial infection        PubMed ID:

トップへページトップへ
利用者成果(日)



戻る 戻る 理研トップページへ