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RCB0007 : HeLa  update : 2018/07/06
CommentJust like ATCC CCL 2, HeLa
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Soeda, Eiichi
Originator Gey, G. O. et al.
Year of deposit 1986
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervix
Case history cervical carcinoma
Classification cancer
Year of origin 1952
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 0
User's Publication 60

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User's Publication
Akita, Hidetaka, Tanimoto, Mitsuhide, Masuda, Tomoya, Kogure, Kentaro, Hama, Susumu, Ninomiya, Keiko, Futaki, Shiroh, Harashima, Hideyoshi  Evaluation of the nuclear delivery and intra-nuclear transcription of plasmid DNA condensed with micro (mu) and NLS-micro by cytoplasmic and nuclear microinjection: a comparative study with poly-L-lysine.  J Gene Med  2006  8:198-206  PubMed ID: 16285003
Bonneau B, Ando H, Kawaai K, Hirose M, Takahashi-Iwanaga H, Mikoshiba K.  IRBIT controls apoptosis by interacting with the Bcl-2 homolog, Bcl2l10, and by promoting ER-mitochondria contact.  Elife  2016  5:  PubMed ID: 27995898
Ueyama T, Ninoyu Y, Nishio SY, Miyoshi T, Torii H, Nishimura K, Sugahara K, Sakata H, Thumkeo D, Sakaguchi H, Watanabe N, Usami SI, Saito N, Kitajiri SI.  Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss.  EMBO Mol Med  2016  8:1310-1324  PubMed ID: 27707755
Kanda S, Yanagitani K, Yokota Y, Esaki Y, Kohno K.  Autonomous translational pausing is required for XBP1u mRNA recruitment to the ER via the SRP pathway.  Proc. Natl. Acad. Sci. U.S.A.  2016  113:E5886-E5895  PubMed ID: 27651490
Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, Kataoka K, Saito T, Chung UI, Ohba S.  Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment.  Sci Rep  2016  6:18743  PubMed ID: 26728350
Bannai H, Niwa F, Sherwood MW, Shrivastava AN, Arizono M, Miyamoto A, Sugiura K, Levi S, Triller A, Mikoshiba K.  Bidirectional Control of Synaptic GABAAR Clustering by Glutamate and Calcium.  Cell Rep  2015  13:2768-80  PubMed ID: 26711343
Abe N, Matsumoto K, Nishihara M, Nakano Y, Shibata A, Maruyama H, Shuto S, Matsuda A, Yoshida M, Ito Y, Abe H.  Rolling Circle Translation of Circular RNA in Living Human Cells.  Sci Rep  2015  5:16435  PubMed ID: 26553571
Kobayashi S, Koujin T, Kojidani T, Osakada H, Mori C, Hiraoka Y, Haraguchi T.  BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy.  Proc. Natl. Acad. Sci. U.S.A.  2015  112:7027-32  PubMed ID: 25991860
Kondo T, Isoda R, Uchimura T, Sugiyama M, Hamao K, Hosoya H.  Diphosphorylated but not monophosphorylated myosin II regulatory light chain localizes to the midzone without its heavy chain during cytokinesis.  Biochem. Biophys. Res. Commun.  2011  417:686-91  PubMed ID: 22166199
Satow R, Shitashige M, Jigami T, Fukami K, Honda K, Kitabayashi I, Yamada T.  β-catenin inhibits promyelocytic leukemia protein tumor suppressor function in colorectal cancer cells.  Gastroenterology  2011  142:572-81  PubMed ID: 22155184
Ochiai K, Yoshikawa Y, Yoshimatsu K, Oonuma T, Tomioka Y, Takeda E, Arikawa J, Mominoki K, Omi T, Hashizume K, Morimatsu M.  Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51.  FEBS Lett.  2011  585:1771-7  PubMed ID: 21601571
Okano K, Yu D, Matsui A, Maezawa Y, Hosokawa Y, Kira A, Matsubara M, Liau I, Tsubokawa H, Masuhara H.  Induction of cell-cell connections by using in situ laser lithography on a perfluoroalkyl-coated cultivation platform.  Chembiochem  2011  12:795-801  PubMed ID: 21341350
Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550
Watanabe S, Wakasugi K.  Module M1 of zebrafish neuroglobin acts as a structural and functional protein building block for a cell-membrane-penetrating activity.  PLoS ONE  2011  6:e16808  PubMed ID: 21304818
Kondo T, Hamao K, Kamijo K, Kimura H, Morita M, Takahashi M, Hosoya H.  Enhancement of myosin II/actin turnover at the contractile ring induces slower furrowing in dividing HeLa cells.  Biochem. J.  2011  435:569-76  PubMed ID: 21231914
Kaneko S, Nakayama H, Yoshino Y, Fushimi D, Yamaguchi K, Horiike Y, Nakanishi J.  Photocontrol of cell adhesion on amino-bearing surfaces by reversible conjugation of poly(ethylene glycol) via a photocleavable linker.  Phys Chem Chem Phys  2011  13:4051-9  PubMed ID: 21225032
Konno Y, Aoki M, Takagishi M, Sakai N, Koike M, Wakamatsu K, Hosoi S.  Enhancement of antibody production by the addition of Coenzyme-Q(10).  Cytotechnology  2011  63:163-70  PubMed ID: 21197574
Hakoda M, Wakizaka Y, Hirota Y.  Separation of viable and nonviable animal cell using dielectrophoretic filter.  Biotechnol. Prog.  2010  26:1061-7  PubMed ID: 20205163
Li F, Awale S, Tezuka Y, Kadota S.  Cytotoxic constituents of propolis from Myanmar and their structure-activity relationship.  Biol. Pharm. Bull.  2009  32:2075-8  PubMed ID: 19952433
Awale S, Miyamoto T, Linn TZ, Li F, Win NN, Tezuka Y, Esumi H, Kadota S.  Cytotoxic constituents of Soymida febrifuga from Myanmar.  J. Nat. Prod.  2009  72:1631-6  PubMed ID: 19689125
Kimura T, Hashimoto I, Nishikawa M, Yamada H.  Nucleocytoplasmic transport of luciferase gene mRNA requires CRM1/Exportin1 and RanGTPase.  Med Mol Morphol  2009  42:70-81  PubMed ID: 19536614
Asano S, Hamao K, Hosoya H.  Direct evidence for roles of phosphorylated regulatory light chain of myosin II in furrow ingression during cytokinesis in HeLa cells.  Genes Cells  2009  14:555-68  PubMed ID: 19371382
Hamao K, Morita M, Hosoya H.  New function of the proline rich domain in dynamin-2 to negatively regulate its interaction with microtubules in mammalian cells.  Exp. Cell Res.  2009  315:1336-45  PubMed ID: 19331814
Yamamoto N, Takemori Y, Sakurai M, Sugiyama K, Sakurai H.  Differential recognition of heat shock elements by members of the heat shock transcription factor family.  FEBS J.  2009  276:1962-74  PubMed ID: 19250318
Koichi Baba, Hitoshi Kasai, Akito Masuhara, Hidetoshi Oikawa, and Hachiro Nakanishi  Organic Solvent-Free Fluorescence Confocal Imaging of Living Cells Using Pure Nanocrystal Forms of Fluorescent Dyes  Jpn J Appl Phys.  2009  48:117002-1-117002-4 
Watanabe S, Wakasugi K.  Zebrafish neuroglobin is a cell-membrane-penetrating globin.  Biochemistry  2008  47:5266-70  PubMed ID: 18416560
Wakasugi K.  Human tryptophanyl-tRNA synthetase binds with heme to enhance its aminoacylation activity.  Biochemistry  2007  46:11291-8  PubMed ID: 17877375
Engelhardt P, Merilainen J, Zhao F, Uchiyama S, Fukui K, Lehto VP.  Whole-mount immunoelectron tomography of chromosomes and cells.  Methods Mol. Biol.  2007  369:387-405  PubMed ID: 17656761
Nakayama T, Nomura N, Matsumura M.  The effect of copper concentration on the virulence of pathogenic Vibrio harveyi.  J. Appl. Microbiol.  2007  102:1300-6  PubMed ID: 17448165
Sasaki Y, Matsui K, Aoyama Y, Kikuchi J.  Cerasome as an infusible and cell-friendly gene carrier: synthesis of cerasome-forming lipids and transfection using cerasome.  Nat Protoc  2007  1:1227-34  PubMed ID: 17406406
Tanaka T, Takahashi M, Kusano E, Okamoto H.  Development and evaluation of an efficient cell-culture system for Hepatitis E virus.  J. Gen. Virol.  2007  88:903-11  PubMed ID: 17325363
Miyauchi K, Yamamoto Y, Kosaka T, Hosoya H.  Myosin II activity is not essential for recruitment of myosin II to the furrow in dividing HeLa cells.  Biochem. Biophys. Res. Commun.  2006  350:543-8  PubMed ID: 17022938
Horiuchi S, Aoyama Y.  Systematic lactose-functionalization of amphiphilic octaamine macrocycle as a gene carrier. Optimization of the charge, size, toxicity, and receptor factors for hepatocyte targeting.  J Control Release  2006  116:107-14  PubMed ID: 16905218
Nakayama T, Nomura N, Matsumura M.  Study on the relationship of protease production and luminescence in Vibrio harveyi.  J. Appl. Microbiol.  2006  101:200-5  PubMed ID: 16834607
Nakayama T, Ito E, Nomura N, Nomura N, Matsumura M.  Comparison of Vibrio harveyi strains isolated from shrimp farms and from culture collection in terms of toxicity and antibiotic resistance.  FEMS Microbiol. Lett.  2006  258:194-9  PubMed ID: 16640572
Matsui K, Horiuchi S, Sando S, Sera T, Aoyama Y.  RNAi silencing of exogenous and endogenous reporter genes using a macrocyclic octaamine as a "compact" siRNA carrier. Studies on the nonsilenced residual activity.  Bioconjug. Chem.  2006  17:132-8  PubMed ID: 16417261
Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, Ito S, Kurosawa K, Yamaguchi K, Sugiura Y, Takizaki H, Yamashita Y, Katakura R, Sato I, Kawai M, Okada Y, Watanabe H, Kondoh G, Matsumoto S, Kishimoto A, Obata M, Matsumoto M, Fukuhara T, Motohashi H, Suematsu M, Komatsu M, Nakayama KI, Watanabe T, Soga T, Shima H, Maemondo M, Tanuma N.  PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.  Cancer Cell  2018  33:355-367.e7  PubMed ID: 29533781
Yoshida W, Saikyo H, Nakabayashi K, Yoshioka H, Bay DH, Iida K, Kawai T, Hata K, Ikebukuro K, Nagasawa K, Karube I.  Identification of G-quadruplex clusters by high-throughput sequencing of whole-genome amplified products with a G-quadruplex ligand.  Sci Rep  2018  8:3116  PubMed ID: 29449667
Hane M, Kitajima K, Sato C.  Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products.  Biochem. Biophys. Res. Commun.  2016  478:1123-9  PubMed ID: 27565727
Lothrop BB, Mulla MS.  Mode of existence and seasonality of midge larvae (Diptera: Chironomidae) in man-made lakes in the Coachella Valley, southern California.  J. Am. Mosq. Control Assoc.  1995  11:77-85  PubMed ID: 7616195
Niwa F, Sakuragi S, Kobayashi A, Takagi S, Oda Y, Bannai H, Mikoshiba K.  Dissection of local Ca(2+) signals inside cytosol by ER-targeted Ca(2+) indicator.  Biochem. Biophys. Res. Commun.  2016  479:67-73  PubMed ID: 27616195
Hozumi Y, Fujiwara H, Kaneko K, Fujii S, Topham MK, Watanabe M, Goto K.  Diacylglycerol kinase ε localizes to subsurface cisterns of cerebellar Purkinje cells.  Cell Tissue Res.  2017  368:441-458  PubMed ID: 28191598
Ono T, Sakamoto C, Nakao M, Saitoh N, Hirano T.  Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ.  Mol. Biol. Cell  2017  28:2875-2886  PubMed ID: 28835373
Goto M, Osada S, Imagawa M, Nishizuka M.  FAD104, a regulator of adipogenesis, is a novel suppressor of TGF-β-mediated EMT in cervical cancer cells.  Sci Rep  2017  7:16365  PubMed ID: 29180690
Baba Y, Shigemi Z, Hara N, Moriguchi M, Ikeda M, Watanabe T, Fujimuro M.  Arctigenin induces the apoptosis of primary effusion lymphoma cells under conditions of glucose deprivation.  Int. J. Oncol.  2018  52:505-517  PubMed ID: 29207179
Yamamura T, Nozu K, Miyoshi Y, Nakanishi K, Fujimura J, Horinouchi T, Minamikawa S, Mori N, Fujimaru R, Nakanishi K, Ninchoji T, Kaito H, Mariko TI, Morioka I, Matsuo M, Iijima K.  An in vitro splicing assay reveals the pathogenicity of a novel intronic variant in ATP6V0A4 for autosomal recessive distal renal tubular acidosis.  BMC Nephrol  2017  18:353  PubMed ID: 29202719
Tsunoyama TA, Watanabe Y, Goto J, Naito K, Kasai RS, Suzuki KGN, Fujiwara TK, Kusumi A.  Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function.  Nat. Chem. Biol.  2018  14:497-506  PubMed ID: 29610485
Morisaku T, Yui H.  Laser-induced surface deformation microscope for the study of the dynamic viscoelasticity of plasma membrane in a living cell.  Analyst  2018  143:2397-2404  PubMed ID: 29700531
  Clinical potential of DAC-70 sol  Integr Mol Med  2016  3: 645-648 
  Biocompatible CdSe/ZnS quantum dot micelles for long-term cell imaging without alteration to the native structure of the blood plasma protein human serum albumin  RSC Adv  2017  7, 2392-2402 
  Development of a Cartridge-type Cell Culture Device Combined with Poly(N-isopropylacrylamide) Coated Substrates  IEEJ Transactions on Sensors and Micromachines  2017  137, 78-83 
Nagai M, Kato K, Oohara K, Shibata T.  Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation  Micromachines (Basel)  2017  8(12). pii: E350  PubMed ID: 30400543
Tang Q, Onitsuka M, Tabata A, Tomoyasu T, Nagamune H.  Construction of Anti-HER2 Recombinants as Targeting Modules for a Drug-delivery System Against HER2-positive Cells.  Anticancer Res.  2018    PubMed ID: 29970568
Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N.  Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport.  J. Cell Biol.  2018    PubMed ID: 30297389
Mitsuhashi H, Homma S, Beermann ML, Ishimaru S, Takeda H, Yu BK, Liu K, Duraiswamy S, Boyce FM, Miller JB.  Efficient system for upstream mRNA trans-splicing to generate covalent, head-to-tail, protein multimers.  Sci Rep  2019    PubMed ID: 30783185
Bannai H, Hirose M, Niwa F, Mikoshiba K.  Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators.  J Vis Exp  2019    PubMed ID: 30958464
Xu B, Ju Y, Cui Y, Song G, Iwase Y, Hosoi A, Morita Y.  tLyP-1-conjugated Au-nanorod@SiO(2) core-shell nanoparticles for tumor-targeted drug delivery and photothermal therapy.  Langmuir  2014  30:7789-97  PubMed ID: 24921672
Inoue Y, Onodera Y, Ishihara K.  Initial Cell Adhesion onto a Phospholipid Polymer Brush Surface Modified with a Terminal Cell Adhesion Peptide.  ACS Appl Mater Interfaces  2018  10:15250-15257  PubMed ID: 29652126
Ryo Taguchia, Takuya Teraia, Tasuku Uenoa, Toru Komatsua, Kenjiro Hanaokaa, Yasuteru Urano  A protein-coupled fluorescent probe for organelle-specific imaging of Na+  Sensors and Actuators B: Chemical   2018  265, 575-581 
Taketoshi Kajimoto, Shun-ichi Nakamura   Quantitative Analysis of Cargo Density in Single-extracellular Vesicles by Imaging   bio-protocol  2018  8 

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