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RCB0007 : HeLa  update : 2018/07/06
CommentJust like ATCC CCL 2, HeLa
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Soeda, Eiichi
Originator Gey, G. O. et al.
Year of deposit 1986
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervix
Case history cervical carcinoma
Classification cancer
Year of origin 1952
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 70


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Reference

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User's Publication
3389  Akita, Hidetaka, Tanimoto, Mitsuhide, Masuda, Tomoya, Kogure, Kentaro, Hama, Susumu, Ninomiya, Keiko, Futaki, Shiroh, Harashima, Hideyoshi  Evaluation of the nuclear delivery and intra-nuclear transcription of plasmid DNA condensed with micro (mu) and NLS-micro by cytoplasmic and nuclear microinjection: a comparative study with poly-L-lysine.  J Gene Med  2006  8:198-206  PubMed ID: 16285003
7046  Bonneau B, Ando H, Kawaai K, Hirose M, Takahashi-Iwanaga H, Mikoshiba K.  IRBIT controls apoptosis by interacting with the Bcl-2 homolog, Bcl2l10, and by promoting ER-mitochondria contact.  Elife  2016  5:  PubMed ID: 27995898
7048  Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, Nagai T.  Five colour variants of bright luminescent protein for real-time multicolour bioimaging.  Nat Commun  2016  7:13718  PubMed ID: 27966527
7058  Ueyama T, Ninoyu Y, Nishio SY, Miyoshi T, Torii H, Nishimura K, Sugahara K, Sakata H, Thumkeo D, Sakaguchi H, Watanabe N, Usami SI, Saito N, Kitajiri SI.  Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss.  EMBO Mol Med  2016  8:1310-1324  PubMed ID: 27707755
7060  Kanda S, Yanagitani K, Yokota Y, Esaki Y, Kohno K.  Autonomous translational pausing is required for XBP1u mRNA recruitment to the ER via the SRP pathway.  Proc. Natl. Acad. Sci. U.S.A.  2016  113:E5886-E5895  PubMed ID: 27651490
7090  Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, Kataoka K, Saito T, Chung UI, Ohba S.  Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment.  Sci Rep  2016  6:18743  PubMed ID: 26728350
7100  Bannai H, Niwa F, Sherwood MW, Shrivastava AN, Arizono M, Miyamoto A, Sugiura K, Levi S, Triller A, Mikoshiba K.  Bidirectional Control of Synaptic GABAAR Clustering by Glutamate and Calcium.  Cell Rep  2015  13:2768-80  PubMed ID: 26711343
7154  Abe N, Matsumoto K, Nishihara M, Nakano Y, Shibata A, Maruyama H, Shuto S, Matsuda A, Yoshida M, Ito Y, Abe H.  Rolling Circle Translation of Circular RNA in Living Human Cells.  Sci Rep  2015  5:16435  PubMed ID: 26553571
7339  Kobayashi S, Koujin T, Kojidani T, Osakada H, Mori C, Hiraoka Y, Haraguchi T.  BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy.  Proc. Natl. Acad. Sci. U.S.A.  2015  112:7027-32  PubMed ID: 25991860
7614  Kondo T, Isoda R, Uchimura T, Sugiyama M, Hamao K, Hosoya H.  Diphosphorylated but not monophosphorylated myosin II regulatory light chain localizes to the midzone without its heavy chain during cytokinesis.  Biochem. Biophys. Res. Commun.  2011  417:686-91  PubMed ID: 22166199
7617  Satow R, Shitashige M, Jigami T, Fukami K, Honda K, Kitabayashi I, Yamada T.  β-catenin inhibits promyelocytic leukemia protein tumor suppressor function in colorectal cancer cells.  Gastroenterology  2011  142:572-81  PubMed ID: 22155184
7739  Koike M, Yutoku Y, Koike A.  Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation.  Biochem. Biophys. Res. Commun.  2011  412:39-43  PubMed ID: 21787748
7809  Ochiai K, Yoshikawa Y, Yoshimatsu K, Oonuma T, Tomioka Y, Takeda E, Arikawa J, Mominoki K, Omi T, Hashizume K, Morimatsu M.  Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51.  FEBS Lett.  2011  585:1771-7  PubMed ID: 21601571
7923  Okano K, Yu D, Matsui A, Maezawa Y, Hosokawa Y, Kira A, Matsubara M, Liau I, Tsubokawa H, Masuhara H.  Induction of cell-cell connections by using in situ laser lithography on a perfluoroalkyl-coated cultivation platform.  Chembiochem  2011  12:795-801  PubMed ID: 21341350
7953  Watanabe S, Wakasugi K.  Module M1 of zebrafish neuroglobin acts as a structural and functional protein building block for a cell-membrane-penetrating activity.  PLoS ONE  2011  6:e16808  PubMed ID: 21304818
7983  Kondo T, Hamao K, Kamijo K, Kimura H, Morita M, Takahashi M, Hosoya H.  Enhancement of myosin II/actin turnover at the contractile ring induces slower furrowing in dividing HeLa cells.  Biochem. J.  2011  435:569-76  PubMed ID: 21231914
7995  Kaneko S, Nakayama H, Yoshino Y, Fushimi D, Yamaguchi K, Horiike Y, Nakanishi J.  Photocontrol of cell adhesion on amino-bearing surfaces by reversible conjugation of poly(ethylene glycol) via a photocleavable linker.  Phys Chem Chem Phys  2011  13:4051-9  PubMed ID: 21225032
8341  Hakoda M, Wakizaka Y, Hirota Y.  Separation of viable and nonviable animal cell using dielectrophoretic filter.  Biotechnol. Prog.  2010  26:1061-7  PubMed ID: 20205163
8436  Li F, Awale S, Tezuka Y, Kadota S.  Cytotoxic constituents of propolis from Myanmar and their structure-activity relationship.  Biol. Pharm. Bull.  2009  32:2075-8  PubMed ID: 19952433
8509  Awale S, Miyamoto T, Linn TZ, Li F, Win NN, Tezuka Y, Esumi H, Kadota S.  Cytotoxic constituents of Soymida febrifuga from Myanmar.  J. Nat. Prod.  2009  72:1631-6  PubMed ID: 19689125
8605  Asano S, Hamao K, Hosoya H.  Direct evidence for roles of phosphorylated regulatory light chain of myosin II in furrow ingression during cytokinesis in HeLa cells.  Genes Cells  2009  14:555-68  PubMed ID: 19371382
8606  Hamao K, Morita M, Hosoya H.  New function of the proline rich domain in dynamin-2 to negatively regulate its interaction with microtubules in mammalian cells.  Exp. Cell Res.  2009  315:1336-45  PubMed ID: 19331814
8644  Yamamoto N, Takemori Y, Sakurai M, Sugiyama K, Sakurai H.  Differential recognition of heat shock elements by members of the heat shock transcription factor family.  FEBS J.  2009  276:1962-74  PubMed ID: 19250318
8677  Koichi Baba, Hitoshi Kasai, Akito Masuhara, Hidetoshi Oikawa, and Hachiro Nakanishi  Organic Solvent-Free Fluorescence Confocal Imaging of Living Cells Using Pure Nanocrystal Forms of Fluorescent Dyes  Jpn J Appl Phys.  2009  48:117002-1-117002-4 
8759  Watanabe S, Wakasugi K.  Zebrafish neuroglobin is a cell-membrane-penetrating globin.  Biochemistry  2008  47:5266-70  PubMed ID: 18416560
8791  Wakasugi K.  Human tryptophanyl-tRNA synthetase binds with heme to enhance its aminoacylation activity.  Biochemistry  2007  46:11291-8  PubMed ID: 17877375
8808  Nakayama T, Nomura N, Matsumura M.  The effect of copper concentration on the virulence of pathogenic Vibrio harveyi.  J. Appl. Microbiol.  2007  102:1300-6  PubMed ID: 17448165
8810  Sasaki Y, Matsui K, Aoyama Y, Kikuchi J.  Cerasome as an infusible and cell-friendly gene carrier: synthesis of cerasome-forming lipids and transfection using cerasome.  Nat Protoc  2007  1:1227-34  PubMed ID: 17406406
8856  Miyauchi K, Yamamoto Y, Kosaka T, Hosoya H.  Myosin II activity is not essential for recruitment of myosin II to the furrow in dividing HeLa cells.  Biochem. Biophys. Res. Commun.  2006  350:543-8  PubMed ID: 17022938
8860  Horiuchi S, Aoyama Y.  Systematic lactose-functionalization of amphiphilic octaamine macrocycle as a gene carrier. Optimization of the charge, size, toxicity, and receptor factors for hepatocyte targeting.  J Control Release  2006  116:107-14  PubMed ID: 16905218
8864  Nakayama T, Nomura N, Matsumura M.  Study on the relationship of protease production and luminescence in Vibrio harveyi.  J. Appl. Microbiol.  2006  101:200-5  PubMed ID: 16834607
8880  Nakayama T, Ito E, Nomura N, Nomura N, Matsumura M.  Comparison of Vibrio harveyi strains isolated from shrimp farms and from culture collection in terms of toxicity and antibiotic resistance.  FEMS Microbiol. Lett.  2006  258:194-9  PubMed ID: 16640572
8903  Matsui K, Horiuchi S, Sando S, Sera T, Aoyama Y.  RNAi silencing of exogenous and endogenous reporter genes using a macrocyclic octaamine as a "compact" siRNA carrier. Studies on the nonsilenced residual activity.  Bioconjug. Chem.  2006  17:132-8  PubMed ID: 16417261
9502  Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, Ito S, Kurosawa K, Yamaguchi K, Sugiura Y, Takizaki H, Yamashita Y, Katakura R, Sato I, Kawai M, Okada Y, Watanabe H, Kondoh G, Matsumoto S, Kishimoto A, Obata M, Matsumoto M, Fukuhara T, Motohashi H, Suematsu M, Komatsu M, Nakayama KI, Watanabe T, Soga T, Shima H, Maemondo M, Tanuma N.  PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.  Cancer Cell  2018  33:355-367.e7  PubMed ID: 29533781
9505  Yoshida W, Saikyo H, Nakabayashi K, Yoshioka H, Bay DH, Iida K, Kawai T, Hata K, Ikebukuro K, Nagasawa K, Karube I.  Identification of G-quadruplex clusters by high-throughput sequencing of whole-genome amplified products with a G-quadruplex ligand.  Sci Rep  2018  8:3116  PubMed ID: 29449667
9802  Hane M, Kitajima K, Sato C.  Effects of intronic single nucleotide polymorphisms (iSNPs) of a polysialyltransferase, ST8SIA2 gene found in psychiatric disorders on its gene products.  Biochem. Biophys. Res. Commun.  2016  478:1123-9  PubMed ID: 27565727
9826  Lothrop BB, Mulla MS.  Mode of existence and seasonality of midge larvae (Diptera: Chironomidae) in man-made lakes in the Coachella Valley, southern California.  J. Am. Mosq. Control Assoc.  1995  11:77-85  PubMed ID: 7616195
10018  Niwa F, Sakuragi S, Kobayashi A, Takagi S, Oda Y, Bannai H, Mikoshiba K.  Dissection of local Ca(2+) signals inside cytosol by ER-targeted Ca(2+) indicator.  Biochem. Biophys. Res. Commun.  2016  479:67-73  PubMed ID: 27616195
10024  Matsumura K, Nakata S, Taniguchi K, Ii H, Ashihara E, Kageyama S, Kawauchi A, Yoshiki T.  Depletion of γ-glutamylcyclotransferase inhibits breast cancer cell growth via cellular senescence induction mediated by CDK inhibitor upregulation.  BMC Cancer  2016  16:748  PubMed ID: 27658708
10129  Hozumi Y, Fujiwara H, Kaneko K, Fujii S, Topham MK, Watanabe M, Goto K.  Diacylglycerol kinase ε localizes to subsurface cisterns of cerebellar Purkinje cells.  Cell Tissue Res.  2017  368:441-458  PubMed ID: 28191598
10236  Sudo K, Niikura K, Iwaki K, Kohyama S, Fujiwara K, Doi N.  Human-derived fusogenic peptides for the intracellular delivery of proteins.  J Control Release  2017  255:1-11  PubMed ID: 28385674
10475  Ono T, Sakamoto C, Nakao M, Saitoh N, Hirano T.  Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ.  Mol. Biol. Cell  2017  28:2875-2886  PubMed ID: 28835373
10628  Goto M, Osada S, Imagawa M, Nishizuka M.  FAD104, a regulator of adipogenesis, is a novel suppressor of TGF-β-mediated EMT in cervical cancer cells.  Sci Rep  2017  7:16365  PubMed ID: 29180690
10629  Baba Y, Shigemi Z, Hara N, Moriguchi M, Ikeda M, Watanabe T, Fujimuro M.  Arctigenin induces the apoptosis of primary effusion lymphoma cells under conditions of glucose deprivation.  Int. J. Oncol.  2018  52:505-517  PubMed ID: 29207179
10637  Yamamura T, Nozu K, Miyoshi Y, Nakanishi K, Fujimura J, Horinouchi T, Minamikawa S, Mori N, Fujimaru R, Nakanishi K, Ninchoji T, Kaito H, Mariko TI, Morioka I, Matsuo M, Iijima K.  An in vitro splicing assay reveals the pathogenicity of a novel intronic variant in ATP6V0A4 for autosomal recessive distal renal tubular acidosis.  BMC Nephrol  2017  18:353  PubMed ID: 29202719
10807  Tsunoyama TA, Watanabe Y, Goto J, Naito K, Kasai RS, Suzuki KGN, Fujiwara TK, Kusumi A.  Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function.  Nat. Chem. Biol.  2018  14:497-506  PubMed ID: 29610485
10812  Morisaku T, Yui H.  Laser-induced surface deformation microscope for the study of the dynamic viscoelasticity of plasma membrane in a living cell.  Analyst  2018  143:2397-2404  PubMed ID: 29700531
10913    Clinical potential of DAC-70 sol  Integr Mol Med  2016  3: 645-648 
10924    Biocompatible CdSe/ZnS quantum dot micelles for long-term cell imaging without alteration to the native structure of the blood plasma protein human serum albumin  RSC Adv  2017  7, 2392-2402 
10945    Development of a Cartridge-type Cell Culture Device Combined with Poly(N-isopropylacrylamide) Coated Substrates  IEEJ Transactions on Sensors and Micromachines  2017  137, 78-83 
11011  Nagai M, Kato K, Oohara K, Shibata T.  Pick-and-Place Operation of Single Cell Using Optical and Electrical Measurements for Robust Manipulation  Micromachines (Basel)  2017  8(12). pii: E350  PubMed ID: 30400543
11117  Tang Q, Onitsuka M, Tabata A, Tomoyasu T, Nagamune H.  Construction of Anti-HER2 Recombinants as Targeting Modules for a Drug-delivery System Against HER2-positive Cells.  Anticancer Res.  2018    PubMed ID: 29970568
11158  Matsumoto S, Caliskan N, Rodnina MV, Murata A, Nakatani K.  Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting.  Nucleic Acids Res.  2018    PubMed ID: 30085309
11494  Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N.  Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport.  J. Cell Biol.  2018    PubMed ID: 30297389
11444  Mitsuhashi H, Homma S, Beermann ML, Ishimaru S, Takeda H, Yu BK, Liu K, Duraiswamy S, Boyce FM, Miller JB.  Efficient system for upstream mRNA trans-splicing to generate covalent, head-to-tail, protein multimers.  Sci Rep  2019    PubMed ID: 30783185
11492  Bannai H, Hirose M, Niwa F, Mikoshiba K.  Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators.  J Vis Exp  2019    PubMed ID: 30958464
4056  Xu B, Ju Y, Cui Y, Song G, Iwase Y, Hosoi A, Morita Y.  tLyP-1-conjugated Au-nanorod@SiO(2) core-shell nanoparticles for tumor-targeted drug delivery and photothermal therapy.  Langmuir  2014  30:7789-97  PubMed ID: 24921672
4058  Inoue Y, Onodera Y, Ishihara K.  Initial Cell Adhesion onto a Phospholipid Polymer Brush Surface Modified with a Terminal Cell Adhesion Peptide.  ACS Appl Mater Interfaces  2018  10:15250-15257  PubMed ID: 29652126
4087  Ryo Taguchia, Takuya Teraia, Tasuku Uenoa, Toru Komatsua, Kenjiro Hanaokaa, Yasuteru Urano  A protein-coupled fluorescent probe for organelle-specific imaging of Na+  Sensors and Actuators B: Chemical   2018  265, 575-581 
4089  Taketoshi Kajimoto, Shun-ichi Nakamura   Quantitative Analysis of Cargo Density in Single-extracellular Vesicles by Imaging   bio-protocol  2018  8 
4205  Shintani Y, Hayata-Takano A, Moriguchi K, Nakazawa T, Ago Y, Kasai A, Seiriki K, Shintani N, Hashimoto H.  β-Arrestin1 and 2 differentially regulate PACAP-induced PAC1 receptor signaling and trafficking.  PLoS ONE  2018    PubMed ID: 29734363
4206  Miyamoto T, Mizuno N, Kosaka M, Fujitani Y, Ohno E, Ohtsuka A.  Conclusive Evidence for OCT4 Transcription in Human Cancer Cell Lines: Possible Role of a Small OCT4-Positive Cancer Cell Population.  Stem Cells  2018    PubMed ID: 29770522
4207  Takahashi T, Mine Y, Okamoto T.  2,3-Dimethoxy-5-methyl-p-benzoquinone (Coenzyme Q0) Disrupts Carbohydrate Metabolism of HeLa Cells by Adduct Formation with Intracellular Free Sulfhydryl-Groups, and Induces ATP Depletion and Necrosis.  Biol. Pharm. Bull.  2018    PubMed ID: 30504682
4208  Suzuki T, Yanai Y, Nishigaki N, Nakatsu Y, Tsuzuki T, Kamiya H.  Effects of mismatches distant from the target position on gene correction with a 5'-tailed duplex.  J. Biosci. Bioeng.  2018    PubMed ID: 29361419
4210  Ichimizu S, Watanabe H, Maeda H, Hamasaki K, Nakamura Y, Chuang VTG, Kinoshita R, Nishida K, Tanaka R, Enoki Y, Ishima Y, Kuniyasu A, Kobashigawa Y, Morioka H, Futaki S, Otagiri M, Maruyama T.  Design and tuning of a cell-penetrating albumin derivative as a versatile nanovehicle for intracellular drug delivery.  J Control Release  2018    PubMed ID: 29530390
4478  Omachi K, Kamura M, Teramoto K, Kojima H, Yokota T, Kaseda S, Kuwazuru J, Fukuda R, Koyama K, Matsuyama S, Motomura K, Shuto T, Suico MA, Kai H.  A Split-Luciferase-Based Trimer Formation Assay as a High-throughput Screening Platform for Therapeutics in Alport Syndrome.  Cell Chem Biol  2018  25(5):634-643.e4  PubMed ID: 29526710
4479  Takahashi T, Mine Y, Okamoto T.  Intracellular reduction of coenzyme Q homologues with a short isoprenoid side chain induces apoptosis of HeLa cells.  J. Biochem.  2018  163(4):329-339  PubMed ID: 29319808
4480  Kimura H, Arasaki K, Ohsaki Y, Fujimoto T, Ohtomo T, Yamada J, Tagaya M.  Syntaxin 17 promotes lipid droplet formation by regulating the distribution of acyl-CoA synthetase 3.  J. Lipid Res.  2018  59(5):805-819  PubMed ID: 29549094
4481  Blaise Kimbadi Lombe, Doris Feineis, Virima Mudogo, Reto Brun, Suresh Awale and Gerhard Bringmann  Michellamines A6 and A7, and further mono- and dimeric naphthylisoquinoline alkaloids from a Congolese Ancistrocladus liana and their antiausterity activities against pancreatic cancer cells  RSC Adv  2018  8:5243 
4530  Yuki Hiruta, ORCID logo, Yuki Kanda, Naoya Katsuyama and Hideko Kanazawa  Dual temperature- and pH-responsive polymeric micelle for selective and efficient two-step doxorubicin delivery  RSC Adv.  2017  7, 29540-29549 



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