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Cell No. : Cell Name
HPS4270 : update : 2024/10/22 |
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| Comment | Disease specific iPS cell line derived from a patient : Down syndrome, trisomy 21. HPS4270, HPS4271, HPS4272, HPS4273 were derived from the same patient. | |||
| Comment from the depositor | Disease specific iPS cell line derived from a person with trisomy 21 (Down syndrome, 47,XY,+21). This iPS cells were created using episomal vectors (OCT3/4, SOX2, KLF4, L-MYC, LIN28, p53-shRNA, and EBNA1). | |||
| Terms and conditions | In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. | |||
| Remarks | ||||
| Order Form | Order Form(C-0042.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Year of deposit | 2019 | ||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Race | Japanese | |||
| Gender | Male | |||
| Age at sampling | under 10 years of age | |||
| Tissue | skin | |||
| Disease name | Down syndrome | |||
| Classification | iPS | |||
| Recombinant | recombinant | |||
| Exogene | Oct3/4, Sox2, Klf4, L-Myc, Lin28, p53-shRNA, EBNA1 | |||
| Vector | pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, pCXLE-hUL, pCXWB-EBNA1 | Lifespan | infinite | |
| Morphology | ES-like | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | StemFit AK02N | |||
| Antibiotics | Free | Passage method | TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1 | |
| Coating dish | see Others (2) | Culture information | Passage cell No | 2.6-26x10 4 cells/60 mm dish |
| SC frequency | Subculture : once/5-8 days, Medium Renewal : every day or every other day | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Feeder cells | Free | |||
| Freeze medium | STEM-CELLBANKER | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Others | (1) Use 10μM Y-27632 when thawing and passaging. (2) Add and mix Laminin-511 E8 (iMatrix511) to the cell suspension at thawing (3.2 ug/mL) and passaging (0.4 ug/mL). | |||
| STR(human) | OK | |||
| Reference information | Reference | 1 | ||
| User's Publication | 2 | |||
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