Search
CELL BANK Website

Back Back
Cell No. : Cell Name
HPS1889 :  update : 2023/06/07
CommentDisease specific iPS cell line derived from a patient : Spinocerebellar degeneration (SCA6). Cryopreserved by vitrification method.
Comment from the depositorDisease specific iPS cell line derived from a patient : Spinocerebellar degeneration (SCA6).
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Rep. 2016;17:1482-1490) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 5) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Year of deposit 2016
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 50s
Tissue skin
Disease name Spinocerebellar degeneration (SCA6)
Classification iPS
Recombinant recombinant
Exogene Oct3/4-shp53, Sox2, Klf4, L-Myc, Lin28
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2.5mM GlutaMAX) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 0


To topTop
Reference
4014  Ishida Y, Kawakami H, Kitajima H, Nishiyama A, Sasai Y, Inoue H, Muguruma K.  Vulnerability of Purkinje Cells Generated from Spinocerebellar Ataxia Type 6 Patient-Derived iPSCs.  Cell Rep  2016  17:1482-1490  PubMed ID: 27806289   DOI: 10.1016/j.celrep.2016.10.026

To topTop
User's Publication



Back Back Return Top Page