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HPS1005 : 1383D2  update : 2019/03/28
CommentHuman iPS cell line derived from healthy individual. Order Form (C-0042, C-0034).
Comment from the depositor
Restriction (Category)f
Terms and conditions1) The RECIPIENT Scientist belongs to a not-for-profit academic organization (i.e. a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Sci Rep. 2014;4:3594) designated by the DEPOSITOR is required. 3) Attach TERMS and CONDITIONS to MTA (C-0034) .
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Yamanaka, Shinya
Year of deposit 2015
Animal human < Mammals
Genus Homo
Species sapiens
Race Asian
Gender Male
Age at sampling 36
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives StemFit AK02N
Antibiotics Free
Passage method 50% TrypLE Select CTS + 0.75mM EDTA + PBS(-)
Coating dish 0.5 μg/cm^(2) Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10^(4) cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium StemFit AK02N + 10 μM Y-27632 + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XY[19], 46,XY,add(4)(q35)[1]
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
Reference information Reference 1
User's Publication 1


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Reference
4002  Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.  A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.  Sci Rep  2014  4:3594  PubMed ID: 24399248

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User's Publication
4048  Shibata S, Hayashi R, Okubo T, Kudo Y, Katayama T, Ishikawa Y, Toga J, Yagi E, Honma Y, Quantock AJ, Sekiguchi K, Nishida K.  Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages  Cell Rep  2018  25(6):1668-1679  PubMed ID: 30404017



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