Cell No. : Cell Name
HPS1005 : 1383D2
update : 2024/02/28
|
| Comment | Human iPS cell line derived from a healthy individual. |
| Comment from the depositor | |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) . |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Yamanaka, Shinya
|
| Year of deposit |
2015
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Asian
|
| Gender |
Male
|
| Age at sampling |
36
|
| Tissue |
peripheral blood
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_UP38
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
StemFit AK02N
|
| Antibiotics |
|
Free
|
|
Passage method |
|
TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
|
| Coating dish |
|
0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
|
Culture information
|
Passage cell No |
|
2.6-26x10 4 cells/60 mm dish
|
| SC frequency |
|
Subculture : once/5-8 days, Medium Renewal : every day or every other day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
Free
|
| Freeze medium |
|
StemFit AK02N + 10 μM Y-27632 + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| Chromosome mode |
|
46,XY[19], 46,XY,add(4)(q35)[1]
|
| Others |
|
Use 10μM Y-27632 when passaging and thawing. Remove it next day.
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
4
|
| User's Publication |
|
19819
Zhang D, Eguchi N, Okazaki S, Sora I, Hishimoto A.
Telencephalon Organoids Derived from an Individual with ADHD Show Altered Neurodevelopment of Early Cortical Layer Structure
Stem Cell Rev Rep
2023
19(5):1482-1491
PubMed ID: 36872412
DOI: 10.1007/s12015-023-10519-z
|
|
21492
Mae SI, Hattanda F, Morita H, Nozaki A, Katagiri N, Ogawa H, Teranaka K, Nishimura Y, Kudoh A, Yamanaka S, Matsuse K, Ryosaka M, Watanabe A, Soga T, Nishio S, Osafune K.
Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
Cell Rep
2023
113431
PubMed ID: 38039961
DOI: 10.1016/j.celrep.2023.113431
|
|
13374
Chang PH, Chao HM, Chern E, Hsu SH.
Chitosan 3D cell culture system promotes naïve-like features of human induced pluripotent stem cells: A novel tool to sustain pluripotency and facilitate differentiation.
Biomaterials
2021
PubMed ID: 33341735
DOI: 10.1016/j.biomaterials.2020.120575
|
|
4048
Shibata S, Hayashi R, Okubo T, Kudo Y, Katayama T, Ishikawa Y, Toga J, Yagi E, Honma Y, Quantock AJ, Sekiguchi K, Nishida K.
Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages
Cell Rep
2018
25(6):1668-1679
PubMed ID: 30404017
DOI: 10.1016/j.celrep.2018.10.032
|
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