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Cell No. : Cell Name
HPS0381 : 1231A3  update : 2024/03/27
CommentHuman iPS cell line derived from a healthy individual. HPS0379, HPS0380, HPS0381, HPS4293 was derived from the same person.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) .
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamanaka, Shinya
Year of deposit 2013
Animal _human < Mammals
Genus Homo
Species sapiens
Race African/American
Gender Female
Age at sampling 29
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_LJ39
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm 2 Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10 4 cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium StemFit AK02N + 10 μM Y-27632 + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XX,inv(9)(p12q13) 46,XX,inv(9)(p12q13) [20] at passage No. 14
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 14


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Reference
20937  Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.  In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.  PLoS One  2022  17(11)6:e0275600  PubMed ID: 36378656   DOI: 10.1371/journal.pone.0275600
4002  Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.  A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.  Sci Rep  2014  4:3594  PubMed ID: 24399248   DOI: 10.1038/srep03594

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User's Publication
21586  Oonishi T, Nishimura K, Takata K, Fujimuro M, Sekine Y.  Scraping Assay as a Novel Strategy to Evaluate Axonal Regeneration Using Human-Induced Pluripotent Stem Cell-Derived Neurons.  Biol Pharm Bull  2024  47(2):366-372  PubMed ID: 38325853   DOI: 10.1248/bpb.b23-00675
21612  Gima S, Oe K, Nishimura K, Ohgita T, Ito H, Kimura H, Saito H, Takata K.  Host-to-graft propagation of inoculated α-synuclein into transplanted human induced pluripotent stem cell-derived midbrain dopaminergic neurons.  Regen Ther  2024  25:229-237  PubMed ID: 38283940   DOI: 10.1016/j.reth.2023.12.019
17772  Lin W, Shiomoto S, Yamada S, Watanabe H, Kawashima Y, Eguchi Y, Muramatsu K, Sekino Y.  Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons  iScience  2023  26(4):106285  PubMed ID: 37034988   DOI: 10.1016/j.isci.2023.106285
21050  Fukuda A, Nakashima S, Oda Y, Nishimura K, Kawashima H, Kimura H, Ohgita T, Kawashita E, Ishihara K, Hanaki A, Okazaki M, Matsuda E, Tanaka Y, Nakamura S, Matsumoto T, Akiba S, Saito H, Matsuda H, Takata K.  Plantainoside B in Bacopa monniera Binds to Aβ Aggregates Attenuating Neuronal Damage and Memory Deficits Induced by Aβ.  Biol Pharm Bull  2023  46(2):320-333  PubMed ID: 36724960   DOI: 10.1248/bpb.b22-00797
21085  Inoue S, Nishimura K, Gima S, Nakano M, Takata K.  CRISPR-Cas9-Edited SNCA Knockout Human Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons and Their Vulnerability to Neurotoxicity.  Biol Pharm Bull  2023  46(3):517-522  PubMed ID: 36858582   DOI: 10.1248/bpb.b22-00839
21123  Takata M, Nishimura K, Harada K, Iwasaki R, Ando M, Yamada S, Ginhoux F, Takata K.  Analysis of Aβ-induced neurotoxicity and microglial responses in simple two- and three-dimensional human iPSC-derived cortical culture systems.  Tissue Cell  2023  81:102023  PubMed ID: 36709697   DOI: 10.1016/j.tice.2023.102023
21260  Taga S, Suga H, Nakano T, Kuwahara A, Inoshita N, Kodani Y, Nagasaki H, Sato Y, Tsumura Y, Sakakibara M, Soen M, Miwata T, Ozaki H, Kano M, Watari K, Ikeda A, Yamanaka M, Takahashi Y, Kitamoto S, Kawaguchi Y, Miyata T, Kobayashi T, Sugiyama M, Onoue T, Yasuda Y, Hagiwara D, Iwama S, Tomigahara Y, Kimura T, Arima H.  Generation and purification of ACTH-secreting hPSC-derived pituitary cells for effective transplantation.  Stem Cell Reports  2023    PubMed ID: 37295423   DOI: 10.1016/j.stemcr.2023.05.002
21294  Kato T, Nishimura K, Hirao M, Shimohama S, Takata K.  Expression and role of nicotinic acetylcholine receptors during midbrain dopaminergic neuron differentiation from human induced pluripotent stem cells.  Neuropsychopharmacol Rep  2023    PubMed ID: 37366076   DOI: 10.1002/npr2.12361
21311  Kaneko H, Kaitsuka T, Tomizawa K.  Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells.  Cell Mol Life Sci  2023  80(8):200  PubMed ID: 37421441   DOI: 10.1007/s00018-023-04847-z
21322  Minami S, Matsumoto N, Omori H, Nakamura Y, Tamiya S, Nouda R, Nurdin JA, Yamasaki M, Kotaki T, Kanai Y, Okamoto T, Tachibana T, Ushijima H, Kobayashi T, Sato S.  Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.  Sci Rep  2023  13(1):11610  PubMed ID: 37463955   DOI: 10.1038/s41598-023-38548-1
21492  Mae SI, Hattanda F, Morita H, Nozaki A, Katagiri N, Ogawa H, Teranaka K, Nishimura Y, Kudoh A, Yamanaka S, Matsuse K, Ryosaka M, Watanabe A, Soga T, Nishio S, Osafune K.  Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.  Cell Rep  2023  113431  PubMed ID: 38039961   DOI: 10.1016/j.celrep.2023.113431
21522  Wu Y, Iwasaki K, Hashimoto Y.  Induction of mesenchymal stem cell (MSC) differentiation from iPS cells using MSC medium.  Dent Mater J  2023    PubMed ID: 38008439   DOI: 10.4012/dmj.2023-130
20573  Nakano Y, Susa K, Yanagi T, Hiraoka Y, Suzuki T, Mori T, Ando F, Mandai S, Fujiki T, Rai T, Uchida S, Sohara E.  Generation of NPHP1 knockout human pluripotent stem cells by a practical biallelic gene deletion strategy using CRISPR/Cas9 and ssODN.  In Vitro Cell Dev Biol Anim  2022    PubMed ID: 35165826   DOI: 10.1007/s11626-022-00655-0
16864  Kenzo BAMBA, Midori OZAWA, Miho TAMAI, Emiko SHIMBO, Hiromasa SHINDO, Takuo OTA, Sungho AHN, Arihiro KOHARA, Yoh-ichi TAGAWA  Human serum albumin-containing xeno-free freezing medium optimized for regenerative medicine  Translational and Regulatory Sciences  2021  3:77-82    DOI: 10.33611/trs.2021-008



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