Cell No. : Cell Name
HPS0328 : 606A1
update : 2024/04/18
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| Comment | Human iPS cell line derived from a healthy individual (cord blood) . HPS0328, HPS0329 was derived from the same person. Cryopreserved by vitrification method. Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Yamanaka, Shinya
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Gender |
Female
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| Tissue |
cord blood
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_DQ11
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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Primate ES Cell Medium + 4ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-5 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.5-2.5 x 10 6 cells/100 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma |
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(-)
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| Virus (HIV) |
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Undetected
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| Virus (HTLV-1) |
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(-)
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| Chromosome mode |
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46XX(20)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
7
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| User's Publication |
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20497
Pircs K, Drouin-Ouellet J, Horváth V, Gil J, Rezeli M, Garza R, Grassi DA, Sharma Y, St-Amour I, Harris K, Jönsson ME, Johansson PA, Vuono R, Fazal SV, Stoker T, Hersbach BA, Sharma K, Lagerwall J, Lagerström S, Storm P, Hébert SS, Marko-Varga G, Parmar M, Barker RA, Jakobsson J.
Distinct subcellular autophagy impairments in induced neurons from Huntington's disease patients.
Brain
2022
145(9):3035-3057
PubMed ID: 34936701
DOI: 10.1093/brain/awab473
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16702
Song D, Takahashi G, Zheng YW, Matsuo-Takasaki M, Li J, Takami M, An Y, Hemmi Y, Miharada N, Fujioka T, Noguchi M, Nakajima T, Saito MK, Nakamura Y, Oda T, Miyaoka Y, Hayashi Y.
Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes
Hum Mol Genet
2022
Apr 7;ddac080
PubMed ID: 35388883
DOI: 10.1093/hmg/ddac080
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12074
Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.
Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.
Heliyon
2020
PubMed ID: 32154424
DOI: 10.1016/j.heliyon.2020.e03493
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13370
Xu L, Hanamatsu H, Homan K, Onodera T, Miyazaki T, Furukawa JI, Hontani K, Tian Y, Baba R, Iwasaki N.
Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes.
Biomolecules
2020
PubMed ID: 33271874
DOI: 10.3390/biom10121622
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13850
Yamashita M, Aoki H, Hashita T, Iwao T, Matsunaga T.
Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells
Fluids Barriers CNS
2020
17(1):36
PubMed ID: 32456699
DOI: 10.1186/s12987-020-00197-1
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4572
Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.
SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.
Nat Commun
2019
10(1):2175
PubMed ID: 31092818
DOI: 10.1038/s41467-019-09511-4
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13851
Grassi DA, Brattås PL, Jönsson ME, Atacho D, Karlsson O, Nolbrant S, Parmar M, Jakobsson J.
Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells
Heliyon
2019
6(1):e03067.
PubMed ID: 31909251
DOI: 10.1016/j.heliyon.2019.e03067
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