Cell No. : Cell Name
HPS0223 : Nips-B2
update : 2023/07/11
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| Comment | Human iPS cell line. iPS cells were created using the Sendai virus vector. Past history : atopic asthma. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 3) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS One. 2012;7:e42855.) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Noguchi, Emiko
|
| Originator |
Hamada, Y., Ono, M.
|
| Year of deposit |
2012
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Age at sampling |
43 years
|
| Tissue |
nasal Epithelial cells
|
| Disease name |
atopic asthma
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, c-Myc
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|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_T775
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-5 split
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| SC frequency |
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Subculture : once/3-5 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
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| CO2 concentration |
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3
%
|
| Feeder cells |
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MEF
|
| Feeder treatment |
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X-rays 5000R (or MMC)
|
| Feeder seed cells |
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1.0-1.5 x 10 6 cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma |
|
(-)
|
| Chromosome mode |
|
46XX(20)
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| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
9
|
| User's Publication |
|
17579
Hirata M, Ichiyanagi T, Katoh H, Hashimoto T, Suzuki H, Nitta H, Kawase M, Nakai R, Imamura M, Ichiyanagi K.
Sequence divergence and retrotransposon insertion underlie interspecific epigenetic differences in primates
Mol Biol Evol
2022
39(10):msac208
PubMed ID: 36219870
DOI: 10.1093/molbev/msac208
|
|
20724
Yasui R, Matsui A, Sekine K, Okamoto S, Taniguchi H.
Highly Sensitive Detection of Human Pluripotent Stem Cells by Loop-Mediated Isothermal Amplification.
Stem Cell Rev Rep
2022
PubMed ID: 35661077
DOI: 10.1007/s12015-022-10402-3
|
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13175
Morita N, Onodera S, Nakamura Y, Nakamura T, Takahashi SI, Nomura T, Azuma T.
Keratinocytes from Gorlin Syndrome-induced pluripotent stem cells are resistant against UV radiation.
Med Mol Morphol
2021
54(2):69-78
PubMed ID: 32816116
DOI: 10.1007/s00795-020-00264-4
|
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13411
Yasui R, Sekine K, Yamaguchi K, Furukawa Y, Taniguchi H.
Robust parameter design of human induced pluripotent stem cell differentiation protocols defines lineage-specific induction of anterior-posterior gut tube endodermal cells.
Stem Cells
2021
PubMed ID: 33400835
DOI: 10.1002/stem.3326
|
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15521
Soeda S, Saito R, Fujii A, Tojo S, Tokumura Y, Taniura H.
Abnormal DNA methylation in pluripotent stem cells from a patient with Prader-Willi syndrome results in neuronal differentiation defects
Stem Cell Res
2021
53:102351
PubMed ID: 33895503
DOI: 10.1016/j.scr.2021.102351
|
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12000
Zujur D, Kanke K, Onodera S, Tani S, Lai J, Azuma T, Xin X, Lichtler AC, Rowe DW, Saito T, Tanaka S, Masaki H, Nakauchi H, Chung UI, Hojo H, Ohba S.
Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers.
Regen Ther
2020
PubMed ID: 31988991
DOI: 10.1016/j.reth.2019.12.010
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10593
Hasegawa D, Ochiai-Shino H, Onodera S, Nakamura T, Saito A, Onda T, Watanabe K, Nishimura K, Ohtaka M, Nakanishi M, Kosaki K, Yamaguchi A, Shibahara T, Azuma T.
Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction.
PLoS ONE
2017
12:e0186879
PubMed ID: 29088246
DOI: 10.1371/journal.pone.0186879
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14039
Sato Y, Kobayashi H, Higuchi T, Shimada Y, Ida H, Ohashi T.
Metabolomic Profiling of Pompe Disease-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Reveals That Oxidative Stress Is Associated with Cardiac and Skeletal Muscle Pathology
Stem Cells Transl Med
2017
6(1):31-39
PubMed ID: 28170191
DOI: 10.5966/sctm.2015-0409
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7266
Sato Y, Kobayashi H, Higuchi T, Shimada Y, Era T, Kimura S, Eto Y, Ida H, Ohashi T.
Disease modeling and lentiviral gene transfer in patient-specific induced pluripotent stem cells from late-onset Pompe disease patient.
Mol Ther Methods Clin Dev
2015
2:15023
PubMed ID: 26199952
DOI: 10.1038/mtm.2015.23
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