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HPS0119 : CiRA188Ai-M1  update : 2019/03/28
CommentDisease specific iPS cell line derived from a patient : Chronic infantile neurological cutaneous and articular (CINCA) syndrome (somatic mosaicism). HPS0120 was derived from the same patient. Application consideration. Order Form (C-0042, C-0057, C-0007 or C-0007p).
Comment from the depositor
Restriction (Category)b
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) Contact Us : RIKEN Cell Bank
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor http://www.cira.kyoto-u.ac.jp/
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 10s
Tissue skin
Disease name CINCA syndrome
Classification iPS
Recombinant recombinant
Exogene pMXs-Oct3/4, pMXs-Sox2, pMXs-Klf4, pMXs-c-Myc
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR (human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 0


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Reference
Tanaka T, Takahashi K, Yamane M, Tomida S, Nakamura S, Oshima K, Niwa A, Nishikomori R, Kambe N, Hara H, Mitsuyama M, Morone N, Heuser JE, Yamamoto T, Watanabe A, Sato-Otsubo A, Ogawa S, Asaka I, Heike T, Yamanaka S, Nakahata T, Saito MK.  Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery.  Blood  2012  120:1299-308  PubMed ID: 22723549

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