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HPS0077 : 454E2  update : 2019/09/06
CommentHuman iPS cell line. Application consideration. Order Form (C-0042, C-0034). Please see CiRA, Kyoto University , if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor
Originator Yamanaka, Shinya
Year of deposit 2011
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 16 years
Tissue dental pulp
Classification iPS
Recombinant recombinant
Exogene pCXLE, human Oct3/4, human Sox2, human Klf4, human L-Myc, human Lin28, p53 shRNA
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/3-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR (human) OK
Chromosome mode 46XX(20)
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 0
User's Publication 15

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User's Publication
7022  Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.  CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.  Nat. Methods  2017  14:963-966  PubMed ID: 28892089
7026  Koui Y, Kido T, Ito T, Oyama H, Chen SW, Katou Y, Shirahige K, Miyajima A.  An In?Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells.  Stem Cell Reports  2017  9:490-498  PubMed ID: 28757162
7080  Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K.  Co-ordinated ocular development from human iPS cells and recovery of corneal function.  Nature  2016  531:376-80  PubMed ID: 26958835
7112  Higuchi A, Kao SH, Ling QD, Chen YM, Li HF, Alarfaj AA, Munusamy MA, Murugan K, Chang SC, Lee HC, Hsu ST, Kumar SS, Umezawa A.  Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity.  Sci Rep  2015  5:18136  PubMed ID: 26656754
7213  Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A.  CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.  Stem Cell Reports  2015  5:508-15  PubMed ID: 26365514
9686  Konagaya S, Iwata H.  Reproducible preparation of spheroids of pancreatic hormone positive cells from human iPS cells: An in vitro study.  Biochim. Biophys. Acta  2016  1860:2008-16  PubMed ID: 27180174
10064  Lin HR, Heish CW, Liu CH, Muduli S, Li HF, Higuchi A, Kumar SS, Alarfaj AA, Munusamy MA, Hsu ST, Chen DC, Benelli G, Murugan K, Cheng NC, Wang HC, Wu GJ.  Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods.  Sci Rep  2017  7:40069  PubMed ID: 28071738
10071  Kamao H, Mandai M, Ohashi W, Hirami Y, Kurimoto Y, Kiryu J, Takahashi M.  Evaluation of the Surgical Device and Procedure for Extracellular Matrix-Scaffold-Supported Human iPSC-Derived Retinal Pigment Epithelium Cell Sheet Transplantation.  Invest. Ophthalmol. Vis. Sci.  2017  58:211-220  PubMed ID: 28114582
10093  Homma K, Usui S, Kaneda M.  Knock-in strategy at 3'-end of Crx gene by CRISPR/Cas9 system shows the gene expression profiles during human photoreceptor differentiation.  Genes Cells  2017  22:250-264  PubMed ID: 28124402
10216  Chen YM, Chen LH, Li MP, Li HF, Higuchi A, Kumar SS, Ling QD, Alarfaj AA, Munusamy MA, Chang Y, Benelli G, Murugan K, Umezawa A.  Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs.  Sci Rep  2017  7:45146  PubMed ID: 28332572
10373  Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M.  Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.  Stem Cell Res  2017  23:13-19  PubMed ID: 28666145
10713  Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG.  Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration.  Sci Rep  2018  8:461  PubMed ID: 29323137
11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143
4117  Csobonyeiova M, Polak S, Danisovic L.  Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling.  Exp. Clin. Endocrinol. Diabetes  2018    PubMed ID: 30114722
4551  Hayashi R, Ishikawa Y, Katori R, Sasamoto Y, Taniwaki Y, Takayanagi H, Tsujikawa M, Sekiguchi K, Quantock AJ, Nishida K.  Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells.  Nat Protoc  2017  12(4):683-696  PubMed ID: 28253236

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