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HPS0076 : 409B2  update : 2019/09/06
細胞特性(Comment:英)Human iPS cell line. HPS0002 and HPS0063 are derived from the same person. Order Form (C-0042, C-0034).Please see CiRA, Kyoto University , if you are a researcher at a for-profit organization.
細胞特性(日)ヒト人工多能性幹(iPS)細胞株。HPS0002、HPS0063同一人。エピソーマルベクター (pCXLE)によりヒト6因子(Oct3/4,Sox2,Klf4,L-Myc,Lin28,p53 shRNA)導入。導入遺伝子は残っていない。ガラス化法(Vitrification法)保存。営利機関は、京都大学iPS細胞研究所にお問い合わせください。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach TERMS and CONDITIONS (non-for-profit) or TERMS and CONDITIONS (for-profit) to MTA (C-0007 or C-0007p) .
使用条件(日)1) 非営利機関以外の利用者は事前にiPSアカデミアジャパン株式会社 から確認書を得ること。2) 別紙1 (非営利) または別紙1 (営利) を提供同意書 (C-0003またはC-0003p) に添付すること。
備考(英)
備考(日)
提供申込書類 依頼書C-0041.pdf   同意書(非営利)C-0003.pdf   同意書(営利)C-0003p.pdf  
提供同意書は、使用機関及び使用内容に応じて二種類あります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は別途ご相談(cellbank.brc@riken.jp)を承ります。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 http://www.cira.kyoto-u.ac.jp/
樹立者 Yamanaka, Shinya
寄託日 2011
動物種 human < Mammals
属名 Homo
種名 sapiens
人種 Caucasian
性別 Female
36 years
採取組織 skin
細胞分類 iPS
遺伝子改変 recombinant
外来遺伝子 pCXLE, human Oct3/4, human Sox2, human Klf4, human L-Myc, human Lin28, p53 shRNA
細胞寿命 infinite
細胞形態 ES-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 Primate ES Cell Medium + 4ng/ml human basic FGF
抗生物質 Free
継代方法 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
培養容器のコーティング 0.1% gelatin coated dish
継代密度 1 : 3-5 split
継代・培地交換頻度 Subculture : once/4-6 days, Medium Renewal : every day
培養最適温度 37 ℃
二酸化炭素濃度 5 %
フィーダー細胞 SNL 76/7
フィーダー細胞の処理方法 X-rays 5000R (or MMC)
フィーダー細胞の播種数 1.5-2.5 x 10^(6) cells/100 mm dish
凍結培地 DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
凍結方法 Vitrification
マイコプラズマ (-)
個体識別検査 OK
染色体検査 46XX(20)
画像情報
寄託時情報
ロット情報
その他の細胞情報 寄託時情報ロット情報
SDS_DAP(MEM)_Japanese.pdf
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 29件
利用者成果(日) 0件

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Reference(英)
4005  iPSC-Based Compound Screening and In Vitro Trials Identify a Synergistic Anti-amyloid β Combination for Alzheimer's Disease.  Kondo T, Imamura K, Funayama M, Tsukita K, Miyake M, Ohta A, Woltjen K, Nakagawa M, Asada T, Arai T, Kawakatsu S, Izumi Y, Kaji R, Iwata N, Inoue H.  Cell Rep  2017  21:2304-2312  PubMed ID: 29166618
3676  A more efficient method to generate integration-free human iPS cells.  Okita K1, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S.  Nat Methods  2011  8(5):409-12  PubMed ID: 21460823

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Reference(日)

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利用者成果(英)
7001  Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.  Akiyama T, Sato S, Chikazawa-Nohtomi N, Soma A, Kimura H, Wakabayashi S, Ko SBH, Ko MSH.  Sci Rep  2018  8:1189  PubMed ID: 29352121
7009  Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells.  Yokoi T, Tanaka T, Matsuzaka E, Tamalu F, Watanabe SI, Nishina S, Azuma N.  Sci Rep  2017  7:16757  PubMed ID: 29196712
7015  Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.  Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.  Stem Cell Reports  2017  9:1441-1449  PubMed ID: 29107592
7026  An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells.  Koui Y, Kido T, Ito T, Oyama H, Chen SW, Katou Y, Shirahige K, Miyajima A.  Stem Cell Reports  2017  9:490-498  PubMed ID: 28757162
7031  Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells.  Ikeda K, Nagata S, Okitsu T, Takeuchi S.  Sci Rep  2017  7:2850  PubMed ID: 28588295
7053  Regeneration of CD8αβ T Cells from T-cell-Derived iPSC Imparts Potent Tumor Antigen-Specific Cytotoxicity.  Maeda T, Nagano S, Ichise H, Kataoka K, Yamada D, Ogawa S, Koseki H, Kitawaki T, Kadowaki N, Takaori-Kondo A, Masuda K, Kawamoto H.  Cancer Res.  2016  76:6839-6850  PubMed ID: 27872100
7213  CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.  Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A.  Stem Cell Reports  2015  5:508-15  PubMed ID: 26365514
9858  Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development.  Mora-Bermúdez F, Badsha F, Kanton S, Camp JG, Vernot B, Köhler K, Voigt B, Okita K, Maricic T, He Z, Lachmann R, Pääbo S, Treutlein B, Huttner WB.  Elife  2016  5:  PubMed ID: 27669147
9918  Matrix-assisted laser desorption/ionization imaging mass spectrometry reveals changes of phospholipid distribution in induced pluripotent stem cell colony differentiation.  Shimizu Y, Satou M, Hayashi K, Nakamura Y, Fujimaki M, Horibata Y, Ando H, Watanabe T, Shiobara T, Chibana K, Takemasa A, Sugimoto H, Anzai N, Ishii Y.  Anal Bioanal Chem  2017  409:1007-1016  PubMed ID: 27815610
9954  Chemoreflex sensitization augments sympathetic vasomotor outflow in awake humans.  Morgan BJ, Crabtree D, Skatrud JB.  Adv. Exp. Med. Biol.  1994  360:269-71  PubMed ID: 7872100
10345  Luteolin attenuates interleukin-6-mediated astrogliosis in human iPSC-derived neural aggregates: A candidate preventive substance for maternal immune activation-induced abnormalities.  Zuiki M, Chiyonobu T, Yoshida M, Maeda H, Yamashita S, Kidowaki S, Hasegawa T, Gotoh H, Nomura T, Ono K, Hosoi H, Morimoto M.  Neurosci. Lett.  2017  653:296-301  PubMed ID: 28595950
10409  Visualization of migration of human cortical neurons generated from induced pluripotent stem cells.  Bamba Y, Kanemura Y, Okano H, Yamasaki M.  J. Neurosci. Methods  2017  289:57-63  PubMed ID: 28694214
10463  Recombinant laminin fragments endowed with collagen-binding activity: A tool for conferring laminin-like cell-adhesive activity to collagen matrices.  Sato-Nishiuchi R, Li S, Ebisu F, Sekiguchi K.  Matrix Biol.  2018  65:75-90  PubMed ID: 28801205
11234  Tumorigenicity-associated characteristics of human iPS cell lines.  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  PLoS ONE  2018    PubMed ID: 30286143
11304  Comparison of the characteristics of mesenchymal stem-like cells derived by integration-free induced pluripotent stem cells in different single-cell culture media under feeder-free conditions.  Ueda M, Hashimoto Y, Honda Y, Baba S, Morita S.  Med Mol Morphol  2018    PubMed ID: 30446810
4124  Approach for differentiating trophoblast cell lineage from human induced pluripotent stem cells with retinoic acid in the absence of bone morphogenetic protein 4.  Ikeda K, Kawasaki Y, Matsuda H, Kishida S, Iwasaki A, Ohata S, Urashima Y, Hirotani Y.  Placenta  2018    PubMed ID: 30415744
4572  SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.  Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.  Nat Commun  2019  10(1):2175  PubMed ID: 31092818
5346  A Human Induced Pluripotent Stem Cell-Derived Tissue Model of a Cerebral Tract Connecting Two Cortical Regions.  Kirihara T, Luo Z, Chow SYA, Misawa R, Kawada J, Shibata S, Khoyratee F, Vollette CA, Volz V, Levi T, Fujii T, Ikeuchi Y.  iScience  2019  14:301-311  PubMed ID: 31006610
5348  Improving the efficiency of gene insertion in a human artificial chromosome vector and its transfer in human-induced pluripotent stem cells  Yoshinori Hasegawa, Masashi Ikeno, Nobutaka Suzuki, Manabu Nakayama, Osamu Ohara  Biology Methods and Protocols  2018  1–10 
5451  Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells.  Tanaka T, Yokoi T, Tamalu F, Watanabe S, Nishina S, Azuma N.  Sci Rep  2015  5:8344  PubMed ID: 25666360
5741  A novel in vitro method for detecting undifferentiated human pluripotent stem cells as impurities in cell therapy products using a highly efficient culture system.  Tano K, Yasuda S, Kuroda T, Saito H, Umezawa A, Sato Y.  PLoS One  2014  9(10):e110496  PubMed ID: 25347300
5774  Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status.  Miyagoe-Suzuki Y, Nishiyama T, Nakamura M, Narita A, Takemura F, Masuda S, Minami N, Murayama K, Komaki H, Goto YI, Takeda S.  Stem Cells Int  2017  2017:7906843  PubMed ID: 28491099
6231  Examination of a Viral Infection Mimetic Model in Human iPS Cell-Derived Insulin-Producing Cells and the Anti-Apoptotic Effect of GLP-1 Analogue.  Baden MY, Fukui K, Hosokawa Y, Iwahashi H, Imagawa A, Shimomura I.  PLoS One  2015  10(12):e0144606  PubMed ID: 26659307
6234  Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells.  Nakamura Y, Shimizu Y, Horibata Y, Tei R, Koike R, Masawa M, Watanabe T, Shiobara T, Arai R, Chibana K, Takemasa A, Sugimoto H, Ishii Y.  Sci Rep  2017  7(1):9377  PubMed ID: 28839272
11576  RNA Virus-Based Episomal Vector with a Fail-Safe Switch Facilitating Efficient Genetic Modification and Differentiation of iPSCs.  Komatsu Y, Takeuchi D, Tokunaga T, Sakurai H, Makino A, Honda T, Ikeda Y, Tomonaga K.  Mol Ther Methods Clin Dev  2019  14:47-55  PubMed ID: 31309127
11589  Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.  Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.  Biochem. Biophys. Res. Commun.  2019  515(4):672-678  PubMed ID: 31178142
11744  Simultaneous precise editing of multiple genes in human cells.  Riesenberg S, Chintalapati M, Macak D, Kanis P, Maricic T, Pääbo S.  Nucleic Acids Res.  2019    PubMed ID: 31392986
11860  Organoid single-cell genomic atlas uncovers human-specific features of brain development.  Kanton S, Boyle MJ, He Z, Santel M, Weigert A, Sanchís-Calleja F, Guijarro P, Sidow L, Fleck JS, Han D, Qian Z, Heide M, Huttner WB, Khaitovich P, Pääbo S, Treutlein B, Camp JG.  Nature  2019    PubMed ID: 31619793
6879  Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells.  Kasai T, Suga H, Sakakibara M, Ozone C, Matsumoto R, Kano M, Mitsumoto K, Ogawa K, Kodani Y, Nagasaki H, Inoshita N, Sugiyama M, Onoue T, Tsunekawa T, Ito Y, Takagi H, Hagiwara D, Iwama S, Goto M, Banno R, Takahashi J, Arima H.  Cell Rep  2020  30(1):18-24  PubMed ID: 31914385

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