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HPS0076 : 409B2  update : 2019/09/06
CommentHuman iPS cell line. HPS0002 and HPS0063 are derived from the same person. Order Form (C-0042, C-0034).Please see CiRA, Kyoto University , if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor
Originator Yamanaka, Shinya
Year of deposit 2011
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Female
Age at sampling 36 years
Tissue skin
Classification iPS
Recombinant recombinant
Exogene pCXLE, human Oct3/4, human Sox2, human Klf4, human L-Myc, human Lin28, p53 shRNA
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR (human) OK
Chromosome mode 46XX(20)
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 1
User's Publication 17

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4005  Kondo T, Imamura K, Funayama M, Tsukita K, Miyake M, Ohta A, Woltjen K, Nakagawa M, Asada T, Arai T, Kawakatsu S, Izumi Y, Kaji R, Iwata N, Inoue H.  iPSC-Based Compound Screening and In Vitro Trials Identify a Synergistic Anti-amyloid β Combination for Alzheimer's Disease.  Cell Rep  2017  21:2304-2312  PubMed ID: 29166618

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User's Publication
7001  Akiyama T, Sato S, Chikazawa-Nohtomi N, Soma A, Kimura H, Wakabayashi S, Ko SBH, Ko MSH.  Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.  Sci Rep  2018  8:1189  PubMed ID: 29352121
7009  Yokoi T, Tanaka T, Matsuzaka E, Tamalu F, Watanabe SI, Nishina S, Azuma N.  Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells.  Sci Rep  2017  7:16757  PubMed ID: 29196712
7015  Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.  Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.  Stem Cell Reports  2017  9:1441-1449  PubMed ID: 29107592
7026  Koui Y, Kido T, Ito T, Oyama H, Chen SW, Katou Y, Shirahige K, Miyajima A.  An In?Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells.  Stem Cell Reports  2017  9:490-498  PubMed ID: 28757162
7031  Ikeda K, Nagata S, Okitsu T, Takeuchi S.  Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells.  Sci Rep  2017  7:2850  PubMed ID: 28588295
7053  Maeda T, Nagano S, Ichise H, Kataoka K, Yamada D, Ogawa S, Koseki H, Kitawaki T, Kadowaki N, Takaori-Kondo A, Masuda K, Kawamoto H.  Regeneration of CD8αβ T Cells from T-cell-Derived iPSC Imparts Potent Tumor Antigen-Specific Cytotoxicity.  Cancer Res.  2016  76:6839-6850  PubMed ID: 27872100
7213  Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A.  CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.  Stem Cell Reports  2015  5:508-15  PubMed ID: 26365514
9858  Mora-Bermúdez F, Badsha F, Kanton S, Camp JG, Vernot B, Köhler K, Voigt B, Okita K, Maricic T, He Z, Lachmann R, Pääbo S, Treutlein B, Huttner WB.  Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development.  Elife  2016  5:  PubMed ID: 27669147
9918  Shimizu Y, Satou M, Hayashi K, Nakamura Y, Fujimaki M, Horibata Y, Ando H, Watanabe T, Shiobara T, Chibana K, Takemasa A, Sugimoto H, Anzai N, Ishii Y.  Matrix-assisted laser desorption/ionization imaging mass spectrometry reveals changes of phospholipid distribution in induced pluripotent stem cell colony differentiation.  Anal Bioanal Chem  2017  409:1007-1016  PubMed ID: 27815610
9954  Morgan BJ, Crabtree D, Skatrud JB.  Chemoreflex sensitization augments sympathetic vasomotor outflow in awake humans.  Adv. Exp. Med. Biol.  1994  360:269-71  PubMed ID: 7872100
10345  Zuiki M, Chiyonobu T, Yoshida M, Maeda H, Yamashita S, Kidowaki S, Hasegawa T, Gotoh H, Nomura T, Ono K, Hosoi H, Morimoto M.  Luteolin attenuates interleukin-6-mediated astrogliosis in human iPSC-derived neural aggregates: A candidate preventive substance for maternal immune activation-induced abnormalities.  Neurosci. Lett.  2017  653:296-301  PubMed ID: 28595950
10409  Bamba Y, Kanemura Y, Okano H, Yamasaki M.  Visualization of migration of human cortical neurons generated from induced pluripotent stem cells.  J. Neurosci. Methods  2017  289:57-63  PubMed ID: 28694214
10463  Sato-Nishiuchi R, Li S, Ebisu F, Sekiguchi K.  Recombinant laminin fragments endowed with collagen-binding activity: A tool for conferring laminin-like cell-adhesive activity to collagen matrices.  Matrix Biol.  2018  65:75-90  PubMed ID: 28801205
11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143
11304  Ueda M, Hashimoto Y, Honda Y, Baba S, Morita S.  Comparison of the characteristics of mesenchymal stem-like cells derived by integration-free induced pluripotent stem cells in different single-cell culture media under feeder-free conditions.  Med Mol Morphol  2018    PubMed ID: 30446810
4124  Ikeda K, Kawasaki Y, Matsuda H, Kishida S, Iwasaki A, Ohata S, Urashima Y, Hirotani Y.  Approach for differentiating trophoblast cell lineage from human induced pluripotent stem cells with retinoic acid in the absence of bone morphogenetic protein 4.  Placenta  2018    PubMed ID: 30415744
4572  Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.  SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.  Nat Commun  2019  10(1):2175  PubMed ID: 31092818

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