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Cell No. : Cell Name
HPS0074 : K31M28  update : 2023/07/26
CommentDisease specific iPS cell line derived from a patient : Pigmentary degeneration of the retina. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) In case of commercial use or profit-making institution use, the RECIPIENT should secure a prior consent of the use from the depositor. 3) In case necessity of personal information such as clinical finding, the research should be collaborated with the depositor. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 5) There is no limitation in the right of the result. 6) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takahashi, Masayo
Originator Takahashi, Masayo
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 60s
Tissue skin
Disease name Retinitis pigmentosa
Classification iPS
Recombinant recombinant
Exogene pMXs-hOct3/4, pMXs-hSox2, pMXs-hKlf4, pMXs-hc-Myc
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_T838
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium Primate ES Cell Medium + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-6 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7 (or STO)
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 0
User's Publication 0


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