Cell No. : Cell Name
HPS0014 : HiPS-RIKEN-2F
update : 2023/06/07
|
| Comment | Human iPS cell line. Parent cell line of RCB0197 HUC-Fm. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Nakamura, Yukio
|
| Originator |
Fujioka, Tsuyoshi
|
| Year of deposit |
2008
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Tissue |
umbilical cord
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_1Q63
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
|
| Coating dish |
|
0.1% gelatin coated dish
|
Culture information
|
Passage ratio |
|
1 : 2-4 split
|
| SC frequency |
|
Subculture : once/3-5 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
3
%
|
| Feeder cells |
|
MEF
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
|
1.0-1.5 x 10 6 cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Relational File |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
2
|
| User's Publication |
5
|
| Reference |
|
9005
Noriko Itaba 1, Peninah M Wairagu, Natsumi Aramaki, Toshihiro Yasui, Yoshiaki Matsumi, Yohei Kono, Ai N H Phan, Makoto Otsu, Takahiro Kunisada, Yukio Nakamura, Hideyuki Okano, Yangsik Jeong, Goshi Shiota
Nuclear Receptor Gene Alteration in Human Induced Pluripotent Stem Cells With Hepatic Differentiation Propensity
Hepatol Res
2014
44 (14), E408-19
PubMed ID: 24636009
DOI: 10.1111/hepr.12329
|
|
9174
Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.
Establishment of induced pluripotent stem cells from human neonatal tissues.
Hum Cell
2010
23(3):113-8
PubMed ID: 20973836
DOI: 10.1111/j.1749-0774.2010.00091.x
|
| User's Publication |
|
21267
Takahashi J, Sugihara HY, Kato S, Nagata S, Okamoto R, Mizutani T.
Protocol to generate large human intestinal organoids using a rotating bioreactor.
STAR Protoc
2023
4(3):102374
PubMed ID: 37352105
DOI: 10.1016/j.xpro.2023.102374
|
|
21034
Takahashi J, Mizutani T, Sugihara HY, Nagata S, Kato S, Hiraguri Y, Takeoka S, Tsuchiya M, Kuno R, Kakinuma S, Watanabe M, Okamoto R.
Suspension culture in a rotating bioreactor for efficient generation of human intestinal organoids.
Cell Rep Methods
2022
2(11):100337
PubMed ID: 36452871
DOI: 10.1016/j.crmeth.2022.100337
|
|
4054
Miyoshi M, Kakinuma S, Kamiya A, Tsunoda T, Tsuchiya J, Sato A, Kaneko S, Nitta S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Nakauchi H, Asahina Y, Watanabe M.
LIM homeobox 2 promotes interaction between human iPS-derived hepatic progenitors and iPS-derived hepatic stellate-like cells.
Sci Rep
2019
9:2072
PubMed ID: 30765795
DOI: 10.1038/s41598-018-37430-9
|
|
9010
Akihide Kamiya, Kazuya Anzai, Kota Tsuruya, Hiromi Chikada
Culture System of Bile Duct-Like Cystic Structures Derived From Human-Inducible Pluripotent Stem Cells
Methods Mol Biol
2019
1905, 143-153
PubMed ID: 30536097
DOI: 10.1007/978-1-4939-8961-4_13
|
|
5448
Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Miyoshi M, Tsunoda T, Nitta S, Asano Y, Nagata H, Otani S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Nakauchi H, Nishitsuji H, Ujino S, Shimotohno K, Iwamoto M, Watashi K, Wakita T, Watanabe M.
Human induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis B virus.
Sci Rep
2016
6:29358
PubMed ID: 27386799
DOI: 10.1038/srep29358
|
Back
Top
CELL BANK Website
SDS_DAP(MEM)_Japanese.pdf