Search
CELL BANK Website

Back Back
Cell No. : Cell Name
HPS0003 : HiPS-RIKEN-1A  update : 2023/07/27
CommentHuman iPS cell line. Parent cell line of RCB0436 HUC-F2. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditionsIn relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells.
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nakamura, Yukio
Originator Fujioka, Tsuyoshi
Year of deposit 2008
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue umbilical cord
Classification iPS
Recombinant recombinant
Exogene pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_B511
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/3-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.0-1.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
Chromosome mode 46XX(18), 47XX+X(2)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 2
User's Publication 6


To topTop
Reference
9002  Naoki Fujitani , Jun-ichi Furukawa, Kayo Araki, Tsuyoshi Fujioka, Yasuhiro Takegawa, Jinhua Piao, Taiki Nishioka, Tomohiro Tamura, Toshio Nikaido, Makoto Ito, Yukio Nakamura, Yasuro Shinohara  Total Cellular Glycomics Allows Characterizing Cells and Streamlining the Discovery Process for Cellular Biomarkers  Proc Natl Acad Sci U S A   2013  110 (6), 2105-10  PubMed ID: 23345451   DOI: 10.1073/pnas.1214233110
9174  Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.  Establishment of induced pluripotent stem cells from human neonatal tissues.  Hum Cell  2010  23(3):113-8  PubMed ID: 20973836   DOI: 10.1111/j.1749-0774.2010.00091.x

To topTop
User's Publication
21544  Ueki M, Suzuki T, Kato Y.  Large-scale cultivation of human iPS cells in bioreactor with reciprocal mixing.  J Biosci Bioeng  2024    PubMed ID: 38185598   DOI: 10.1016/j.jbiosc.2023.12.008
21546  Kuroda T, Yasuda S, Matsuyama S, Miura T, Sawada R, Matsuyama A, Yamamoto Y, Morioka MS, Kawaji H, Kasukawa T, Itoh M, Akutsu H, Kawai J, Sato Y.  ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons.  Sci Rep  2024  14(1):690  PubMed ID: 38184695   DOI: 10.1038/s41598-023-51082-4
4572  Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.  SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.  Nat Commun  2019  10(1):2175  PubMed ID: 31092818   DOI: 10.1038/s41467-019-09511-4
11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143   DOI: 10.1371/journal.pone.0205022
7562  Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama a, Keiko Tano, Shinji Kusakawa, Yoshiki Sawa, Shin Kawamata, Yoji Sato  Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells.  Regenerative Therapy  2015  2:17-23  PubMed ID: 31245455   DOI: 10.1016/j.reth.2015.08.001
16884  Maeda T, Lee MJ, Palczewska G, Marsili S, Tesar PJ, Palczewski K, Takahashi M, Maeda A.  Retinal pigmented epithelial cells obtained from human induced pluripotent stem cells possess functional visual cycle enzymes in vitro and in viv  J Biol Chem   2013  288(48):34484-9  PubMed ID: 24129572   DOI: 10.1074/jbc.M113.518571



Back Back Return Top Page