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HPS0003 : HiPS-RIKEN-1A  update : 2019/09/06
CommentHuman iPS cell line. Parent cell line of RCB0436 HUC-F2. Order Form (C-0042, C-0007 or C-0007p).
Comment from the depositor
Terms and conditionsIn relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Nakamura, Yukio
Originator Fujioka, Tsuyoshi
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue umbilical cord
Classification iPS
Recombinant recombinant
Exogene pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/3-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.0-1.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Chromosome mode 46XX(18), 47XX+X(2)
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 2
User's Publication 4


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Reference
9002  Naoki Fujitani , Jun-ichi Furukawa, Kayo Araki, Tsuyoshi Fujioka, Yasuhiro Takegawa, Jinhua Piao, Taiki Nishioka, Tomohiro Tamura, Toshio Nikaido, Makoto Ito, Yukio Nakamura, Yasuro Shinohara  Total Cellular Glycomics Allows Characterizing Cells and Streamlining the Discovery Process for Cellular Biomarkers  Proc Natl Acad Sci U S A   2013  110 (6), 2105-10  PubMed ID: 23345451
9174  Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.  Establishment of induced pluripotent stem cells from human neonatal tissues.  Hum Cell  2010  23(3):113-8  PubMed ID: 20973836

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User's Publication
4572  Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.  SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.  Nat Commun  2019  10(1):2175  PubMed ID: 31092818
11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143
7562  Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama a, Keiko Tano, Shinji Kusakawa, Yoshiki Sawa, Shin Kawamata, Yoji Sato  Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells.  Regenerative Therapy  2015  2:17-23 
9008  Tadao Maeda, Mee Jee Lee, Grazyna Palczewska, Stefania Marsili, Paul J Tesar, Krzysztof Palczewski, Masayo Takahashi, Akiko Maeda  Retinal Pigmented Epithelial Cells Obtained From Human Induced Pluripotent Stem Cells Possess Functional Visual Cycle Enzymes in Vitro and in Vivo  J Biol Chem  2013  288 (48), 34484-93  PubMed ID: 24129572



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