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HPS0002 : 253G1  update : 2019/03/28
細胞特性(Comment:英)Human iPS cell line. HPS0063 and HPS0076 were derived from the same patient. Order Form (C-0042, C-0034). Please see CiRA , Kyoto University if you are a researcher at a for-profit organization.
細胞特性(日)ヒト人工多能性幹(iPS)細胞株。HPS0063、HPS0076と同一人由来。レトロウイルスベクターにより3因子(Oct3/4, Sox2, Klf4)を導入して樹立。ガラス化法(Vitrification法)保存。営利機関は、京都大学iPS細胞研究所から配布。
使用条件(英)1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
使用条件(日)1) 利用者は学術機関に所属している者に限る。2) 別紙1を提供同意書 (C-0028) に添付すること。
提供申込書類 依頼書C-0041.pdf   同意書C-0028.pdf  
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 http://www.cira.kyoto-u.ac.jp/
樹立者 Yamanaka, Shinya
寄託日 2008
動物種 human < Mammals
属名 Homo
種名 sapiens
人種 Caucasian
性別 Female
36 years
採取組織 skin
細胞分類 iPS
遺伝子改変 recombinant
外来遺伝子 pMXs, Oct3/4, Sox2, Klf4, pLenti6/UbC/V5-DEST, mouse Slc7a1
細胞寿命 infinite
細胞形態 ES-like
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 Primate ES Cell Medium + 4ng/ml human basic FGF
抗生物質 Free
継代方法 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
培養容器のコーティング 0.1% gelatin coated dish
継代密度 1 : 3-6 split
継代・培地交換頻度 Subculture : once/4-5 days, Medium Renewal : every day
培養最適温度 37 ℃
二酸化炭素濃度 5 %
フィーダー細胞 SNL 76/7
フィーダー細胞の処理方法 X-rays 5000R (or MMC)
フィーダー細胞の播種数 1.5-2.5 x 10^(6) cells/100 mm dish
凍結培地 DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
凍結方法 Vitrification
マイコプラズマ (-)
個体識別検査 OK
染色体検査 46XX(20)
その他の細胞情報 寄託時情報ロット情報
文献情報 Reference(英) 0件
Reference(日) 0件
利用者成果(英) 33件
利用者成果(日) 1件



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7047  Numaga-Tomita T, Kitajima N, Kuroda T, Nishimura A, Miyano K, Yasuda S, Kuwahara K, Sato Y, Ide T, Birnbaumer L, Sumimoto H, Mori Y, Nishida M.  TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis.  Sci Rep  2016  6:39383  PubMed ID: 27991560
7080  Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K.  Co-ordinated ocular development from human iPS cells and recovery of corneal function.  Nature  2016  531:376-80  PubMed ID: 26958835
7083  Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T.  TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.  Sci Rep  2016  6:21747  PubMed ID: 26888607
7271  Fujiwara N, Shimizu J, Takai K, Arimitsu N, Ueda Y, Wakisaka S, Suzuki T, Suzuki N.  Cellular and molecular mechanisms of the restoration of human APP transgenic mouse cognitive dysfunction after transplant of human iPS cell-derived neural cells.  Exp. Neurol.  2015  271:423-31  PubMed ID: 26196079
7518  Kagawa Y, Matsuura K, Shimizu T, Tsuneda S.  Direct measurement of local dissolved oxygen concentration spatial profiles in a cell culture environment.  Biotechnol. Bioeng.  2015  112:1263-74  PubMed ID: 25565074
7562  Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama a, Keiko Tano, Shinji Kusakawa, Yoshiki Sawa, Shin Kawamata, Yoji Sato  Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells.  Regenerative Therapy  2015  2:17-23 
7597  Teramura T, Takehara T, Onodera Y, Nakagawa K, Hamanishi C, Fukuda K.  Mechanical stimulation of cyclic tensile strain induces reduction of pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells.  Biochem. Biophys. Res. Commun.  2011  417:836-41  PubMed ID: 22206673
7771  Ogawa S, Tokumoto Y, Miyake J, Nagamune T.  Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells.  In Vitro Cell. Dev. Biol. Anim.  2011  47:464-9  PubMed ID: 21695581
9539  Amano Y, Nishiguchi A, Matsusaki M, Iseoka H, Miyagawa S, Sawa Y, Seo M, Yamaguchi T, Akashi M.  Development of vascularized iPSC derived 3D-cardiomyocyte tissues by filtration Layer-by-Layer technique and their application for pharmaceutical assays.  Acta Biomater  2016  33:110-21  PubMed ID: 26821339
9814  Suga M, Tachikawa S, Tateyama D, Ohnuma K, Furue MK.  Imaging-cytometry revealed spatial heterogeneities of marker expression in undifferentiated human pluripotent stem cells.  In Vitro Cell. Dev. Biol. Anim.  2017  53:83-91  PubMed ID: 27573412
9838  Tomotsune D, Hirashima K, Fujii M, Yue F, Matsumoto K, Takizawa-Shirasawa S, Yokoyama T, Sasaki K.  Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment.  PLoS ONE  2016  11:e0162693  PubMed ID: 27632182
10071  Kamao H, Mandai M, Ohashi W, Hirami Y, Kurimoto Y, Kiryu J, Takahashi M.  Evaluation of the Surgical Device and Procedure for Extracellular Matrix-Scaffold-Supported Human iPSC-Derived Retinal Pigment Epithelium Cell Sheet Transplantation.  Invest. Ophthalmol. Vis. Sci.  2017  58:211-220  PubMed ID: 28114582
10089  Burden ML, Burden AC.  The American Diabetes Association screening questionnaire for diabetes. Is it worthwhile in the U.K.?  Diabetes Care  1994  17:97  PubMed ID: 8112198
10308  Iseoka H, Miyagawa S, Fukushima S, Saito A, Masuda S, Yajima S, Ito E, Sougawa N, Takeda M, Harada A, Lee JK, Sawa Y.  Pivotal Role of Non-cardiomyocytes in Electromechanical and Therapeutic Potential of Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissue.  Tissue Eng Part A  2018  24:287-300  PubMed ID: 28498040
10426  Tateno H, Saito S.  Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells.  Molecules  2017  22:  PubMed ID: 28698527
10503  Suzuki T, Shimizu Y, Furuhata E, Maeda S, Kishima M, Nishimura H, Enomoto S, Hayashizaki Y, Suzuki H.  RUNX1 regulates site specificity of DNA demethylation by recruitment of DNA demethylation machineries in hematopoietic cells.  Blood Adv  2017  1:1699-1711  PubMed ID: 29296817
10593  Hasegawa D, Ochiai-Shino H, Onodera S, Nakamura T, Saito A, Onda T, Watanabe K, Nishimura K, Ohtaka M, Nakanishi M, Kosaki K, Yamaguchi A, Shibahara T, Azuma T.  Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction.  PLoS ONE  2017  12:e0186879  PubMed ID: 29088246
10737  Kato Y, Kim MH, Kino-Oka M.  Comparison of growth kinetics between static and dynamic cultures of human induced pluripotent stem cells.  J. Biosci. Bioeng.  2018    PubMed ID: 29398548
10891    Human iPS cell derived neural cell sheets exhibit mature neural and extendable scaffold functions and promote recovery in injured mouse spinal cords  J Stem Cell Res Med  2016  1: 41-47 
10933    Impairment of the transition from proliferative stage to prehypertrophic stage in chondrogenic differentiation of human induced pluripotent stem cells harboring the causative mutation of achondroplasia in fibroblast growth factor receptor 3  Regenerative Therapy  2017  6, 15-20  PubMed ID: 30271835
11171  Yamada S, Kubo Y, Yamazaki D, Sekino Y, Nomura Y, Yoshida S, Kanda Y.  Tributyltin Inhibits Neural Induction of Human Induced Pluripotent Stem Cells.  Sci Rep  2018    PubMed ID: 30108368
11235  Yoda K, Ohnuki Y, Kurosawa H.  Optimization of the treatment conditions with glycogen synthase kinase-3 inhibitor towards enhancing the proliferation of human induced pluripotent stem cells while maintaining an undifferentiated state under feeder-free conditions.  J. Biosci. Bioeng.  2018    PubMed ID: 30292671
11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143
11325  Yamada S, Yamazaki D, Kanda Y.  5-Fluorouracil inhibits neural differentiation via Mfn1/2 reduction in human induced pluripotent stem cells.  J Toxicol Sci  2018    PubMed ID: 30518710
11400  Konagaya S, Iwata H.  Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells.  Sci Rep  2019    PubMed ID: 30679498
11424  Dion J, Minoshima F, Saito S, Kiyoi K, Hasehira K, Tateno H.  Photoactivable elimination of tumorigenic human induced pluripotent stem cells using a lectin-doxorubicin prodrug conjugate.  Chembiochem  2019    PubMed ID: 30737871
11482  Matsumoto E, Koide N, Hanzawa H, Kiyama M, Ohta M, Kuwabara J, Takeda S, Takahashi M.  Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine.  PLoS ONE  2019    PubMed ID: 30865653
4069  Tadashi Kosawada, Email author Keita, Ohnishi, Hiroaki Satoh, Zhong-gang Feng, Kaoru Goto  Novel methods to apply micro dynamic stimulations on cultured adhesive cells and its application in constructing gel-embedded three-dimensional neuronal structures differentiated from human iPS cells  Microsystem Technologies  2018  24:625–638 
4116  Uto S, Nishizawa S, Hikita A, Takato T, Hoshi K.  Application of induced pluripotent stem cells for cartilage regeneration in CLAWN miniature pig osteochondral replacement model.  Regen Ther  2018    PubMed ID: 30525076
4117  Csobonyeiova M, Polak S, Danisovic L.  Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling.  Exp. Clin. Endocrinol. Diabetes  2018    PubMed ID: 30114722
4463  Masuda S, Matsuura K, Shimizu T.  Preparation of iPS cell-derived CD31<sup>+</sup> endothelial cells using three-dimensional suspension culture.  Regen Ther  2018  9:1-9  PubMed ID: 30525069
4464  Takeda M, Miyagawa S, Fukushima S, Saito A, Ito E, Harada A, Matsuura R, Iseoka H, Sougawa N, Mochizuki-Oda N, Matsusaki M, Akashi M, Sawa Y.  Development of In Vitro Drug-Induced Cardiotoxicity Assay by Using Three-Dimensional Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells.  Tissue Eng Part C Methods  2018  24(1):56-67  PubMed ID: 28967302

10987    再生医学的手法を用いた認知症に対する新規治療法の開発に向けて  聖マリアンナ医科大学雑誌  2017  45, 21–24 

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