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HPS0002 : 253G1  update : 2019/03/28
CommentHuman iPS cell line. HPS0063 and HPS0076 were derived from the same patient. Order Form (C-0042, C-0034). Please see CiRA , Kyoto University if you are a researcher at a for-profit organization.
Comment from the depositor
Restriction (Category)f
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor http://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Female
Age at sampling 36 years
Tissue skin
Classification iPS
Recombinant recombinant
Exogene pMXs, Oct3/4, Sox2, Klf4, pLenti6/UbC/V5-DEST, mouse Slc7a1
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-6 split
SC frequency Subculture : once/4-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR (human) OK
Chromosome mode 46XX(20)
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 0
User's Publication 30


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Reference

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User's Publication
Liu L, Kamei KI, Yoshioka M, Nakajima M, Li J, Fujimoto N, Terada S, Tokunaga Y, Koyama Y, Sato H, Hasegawa K, Nakatsuji N, Chen Y.  Nano-on-micro fibrous extracellular matrices for scalable expansion of human ES/iPS cells.  Biomaterials  2017  124:47-54  PubMed ID: 28187394
Numaga-Tomita T, Kitajima N, Kuroda T, Nishimura A, Miyano K, Yasuda S, Kuwahara K, Sato Y, Ide T, Birnbaumer L, Sumimoto H, Mori Y, Nishida M.  TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis.  Sci Rep  2016  6:39383  PubMed ID: 27991560
Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K.  Co-ordinated ocular development from human iPS cells and recovery of corneal function.  Nature  2016  531:376-80  PubMed ID: 26958835
Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T.  TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.  Sci Rep  2016  6:21747  PubMed ID: 26888607
Fujiwara N, Shimizu J, Takai K, Arimitsu N, Ueda Y, Wakisaka S, Suzuki T, Suzuki N.  Cellular and molecular mechanisms of the restoration of human APP transgenic mouse cognitive dysfunction after transplant of human iPS cell-derived neural cells.  Exp. Neurol.  2015  271:423-31  PubMed ID: 26196079
Tateno H, Onuma Y, Ito Y, Minoshima F, Saito S, Shimizu M, Aiki Y, Asashima M, Hirabayashi J.  Elimination of tumorigenic human pluripotent stem cells by a recombinant lectin-toxin fusion protein.  Stem Cell Reports  2015  4:811-20  PubMed ID: 25866158
Kagawa Y, Matsuura K, Shimizu T, Tsuneda S.  Direct measurement of local dissolved oxygen concentration spatial profiles in a cell culture environment.  Biotechnol. Bioeng.  2015  112:1263-74  PubMed ID: 25565074
Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama a, Keiko Tano, Shinji Kusakawa, Yoshiki Sawa, Shin Kawamata, Yoji Sato  Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells.  Regenerative Therapy  2015  2:17-23 
Teramura T, Takehara T, Onodera Y, Nakagawa K, Hamanishi C, Fukuda K.  Mechanical stimulation of cyclic tensile strain induces reduction of pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells.  Biochem. Biophys. Res. Commun.  2011  417:836-41  PubMed ID: 22206673
Ogawa S, Tokumoto Y, Miyake J, Nagamune T.  Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells.  In Vitro Cell. Dev. Biol. Anim.  2011  47:464-9  PubMed ID: 21695581
Amano Y, Nishiguchi A, Matsusaki M, Iseoka H, Miyagawa S, Sawa Y, Seo M, Yamaguchi T, Akashi M.  Development of vascularized iPSC derived 3D-cardiomyocyte tissues by filtration Layer-by-Layer technique and their application for pharmaceutical assays.  Acta Biomater  2016  33:110-21  PubMed ID: 26821339
Suga M, Tachikawa S, Tateyama D, Ohnuma K, Furue MK.  Imaging-cytometry revealed spatial heterogeneities of marker expression in undifferentiated human pluripotent stem cells.  In Vitro Cell. Dev. Biol. Anim.  2017  53:83-91  PubMed ID: 27573412
Tomotsune D, Hirashima K, Fujii M, Yue F, Matsumoto K, Takizawa-Shirasawa S, Yokoyama T, Sasaki K.  Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment.  PLoS ONE  2016  11:e0162693  PubMed ID: 27632182
Kamao H, Mandai M, Ohashi W, Hirami Y, Kurimoto Y, Kiryu J, Takahashi M.  Evaluation of the Surgical Device and Procedure for Extracellular Matrix-Scaffold-Supported Human iPSC-Derived Retinal Pigment Epithelium Cell Sheet Transplantation.  Invest. Ophthalmol. Vis. Sci.  2017  58:211-220  PubMed ID: 28114582
Burden ML, Burden AC.  The American Diabetes Association screening questionnaire for diabetes. Is it worthwhile in the U.K.?  Diabetes Care  1994  17:97  PubMed ID: 8112198
Iseoka H, Miyagawa S, Fukushima S, Saito A, Masuda S, Yajima S, Ito E, Sougawa N, Takeda M, Harada A, Lee JK, Sawa Y.  Pivotal Role of Non-cardiomyocytes in Electromechanical and Therapeutic Potential of Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissue.  Tissue Eng Part A  2018  24:287-300  PubMed ID: 28498040
Tateno H, Saito S.  Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells.  Molecules  2017  22:  PubMed ID: 28698527
Suzuki T, Shimizu Y, Furuhata E, Maeda S, Kishima M, Nishimura H, Enomoto S, Hayashizaki Y, Suzuki H.  RUNX1 regulates site specificity of DNA demethylation by recruitment of DNA demethylation machineries in hematopoietic cells.  Blood Adv  2017  1:1699-1711  PubMed ID: 29296817
Hasegawa D, Ochiai-Shino H, Onodera S, Nakamura T, Saito A, Onda T, Watanabe K, Nishimura K, Ohtaka M, Nakanishi M, Kosaki K, Yamaguchi A, Shibahara T, Azuma T.  Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction.  PLoS ONE  2017  12:e0186879  PubMed ID: 29088246
Kato Y, Kim MH, Kino-Oka M.  Comparison of growth kinetics between static and dynamic cultures of human induced pluripotent stem cells.  J. Biosci. Bioeng.  2018    PubMed ID: 29398548
  Human iPS cell derived neural cell sheets exhibit mature neural and extendable scaffold functions and promote recovery in injured mouse spinal cords  J Stem Cell Res Med  2016  1: 41-47 
  Impairment of the transition from proliferative stage to prehypertrophic stage in chondrogenic differentiation of human induced pluripotent stem cells harboring the causative mutation of achondroplasia in fibroblast growth factor receptor 3  Regenerative Therapy  2017  6, 15-20 
Yamada S, Kubo Y, Yamazaki D, Sekino Y, Nomura Y, Yoshida S, Kanda Y.  Tributyltin Inhibits Neural Induction of Human Induced Pluripotent Stem Cells.  Sci Rep  2018    PubMed ID: 30108368
Yoda K, Ohnuki Y, Kurosawa H.  Optimization of the treatment conditions with glycogen synthase kinase-3 inhibitor towards enhancing the proliferation of human induced pluripotent stem cells while maintaining an undifferentiated state under feeder-free conditions.  J. Biosci. Bioeng.  2018    PubMed ID: 30292671
Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143
Yamada S, Yamazaki D, Kanda Y.  5-Fluorouracil inhibits neural differentiation via Mfn1/2 reduction in human induced pluripotent stem cells.  J Toxicol Sci  2018    PubMed ID: 30518710
Konagaya S, Iwata H.  Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells.  Sci Rep  2019    PubMed ID: 30679498
Dion J, Minoshima F, Saito S, Kiyoi K, Hasehira K, Tateno H.  Photoactivable elimination of tumorigenic human induced pluripotent stem cells using a lectin-doxorubicin prodrug conjugate.  Chembiochem  2019    PubMed ID: 30737871
Matsumoto E, Koide N, Hanzawa H, Kiyama M, Ohta M, Kuwabara J, Takeda S, Takahashi M.  Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine.  PLoS ONE  2019    PubMed ID: 30865653
Tadashi Kosawada, Email author Keita, Ohnishi, Hiroaki Satoh, Zhong-gang Feng, Kaoru Goto  Novel methods to apply micro dynamic stimulations on cultured adhesive cells and its application in constructing gel-embedded three-dimensional neuronal structures differentiated from human iPS cells  Microsystem Technologies  2018  24:625–638 



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