Cell No. : Cell Name HEV0031 :  update : 2024/09/30
Comment			Human B lymphocytes transformed by Epstein-Barr Virus. HLA A*02:06 A*02:10, B*39:01 B*40:06, C*07:02 C*08:01, DRB1*14:03 DRB1*15:01, DQB1*03:01 DQB1*06:02, DPB1*02:01 DPB1*05:01
Comment from the depositor
Terms and conditions			1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref.(Mutat Res 2005; 588: 1-6) designated by the DEPOSITOR is required. 2) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0047.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Year of deposit	2004
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	65
		Tissue	peripheral blood lymphocytes
		Classification	transformed
		Memo_1	Mycoplasma was eliminated with MC-210 by the depositor.
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_T075
	HEV related information	Alcohol	No
		Smoking	No smoking
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 20% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 4-6 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		LT 400
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP
		STR(human)		OK
Reference information		Reference	2
		User's Publication	3

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Reference

3601  Iwakawa M, Goto M, Noda S, Sagara M, Yamada S, Yamamoto N, Kawakami Y, Matsui Y, Miyazawa Y, Yamazaki H, Tsuji H, Ohno T, Mizoe J, Tsujii H, Imai T.  DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy volunteers.  Mutat Res  2005  588(1):1-6  PubMed ID: 16260175   DOI: 10.1016/j.mrgentox.2005.07.012

3157  Ban S, Konomi C, Iwakawa M, Yamada S, Ohno T, Tsuji H, Noda S, Matui Y, Harada Y, Cologne JB, Imai T.  Radiosensitivity of peripheral blood lymphocytes obtained from patients with cancers of the breast, head and neck or cervix as determined with a micronucleus assay.  J Radiat Res  2004  5(4):535-41  PubMed ID: 15635263   DOI: 10.1269/jrr.45.535

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User's Publication

13738  Taniguchi I, Iwaya C, Ohnaka K, Shibata H, Yamamoto K.  Genome-wide DNA methylation analysis reveals hypomethylation in the low-CpG promoter regions in lymphoblastoid cell lines  Hum Genomics  2017  11(1):8  PubMed ID: 28499412   DOI: 10.1186/s40246-017-0106-6

7664  Takeuchi F, Yokota M, Yamamoto K, Nakashima E, Katsuya T, Asano H, Isono M, Nabika T, Sugiyama T, Fujioka A, Awata N, Ohnaka K, Nakatochi M, Kitajima H, Rakugi H, Nakamura J, Ohkubo T, Imai Y, Shimamoto K, Yamori Y, Yamaguchi S, Kobayashi S, Takayanagi R, Ogihara T, Kato N.  Genome-wide association study of coronary artery disease in the Japanese.  Eur. J. Hum. Genet.  2012  20:333-40  PubMed ID: 21971053   DOI: 10.1038/ejhg.2011.184

15729  H Kitajima, M Sonoda, K Yamamoto  HLA and SNP haplotype mapping in the Japanese population  Genes Immun  2012  13(7):543-8  PubMed ID: 22914434   DOI: 10.1038/gene.2012.35