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HES0006 : SEES1  update : 2018/07/06
CommentHuman embryonic stem cell line.
Comment from the depositor
Use conditionf
Use condition descriptionPlease read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells .
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2011
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morpholology ES-like
deposit info
lot info
Medium Medium List
Anchordepd Adherent cells
Medium and additives iPSellon + 10ng/ml human basic FGF
Antibiotics within iPSellon (Penicillin, Streptomycin)
Passage method 0.1% Dispase II
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 4-5 x 10^(5) cells/60 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR (human) OK
Chromosome mode 46XX(20)
Images
deposit info
lot info
Reference information Reference 0
User's Publication 0


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