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HES0003 : KhES-3  update : 2018/07/06
CommentHuman embryonic stem cell line.
Comment from the depositor
Use conditionb
Use condition description1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells .
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Nakatsuji, Norio
Originator Nakatsuji, Norio
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morpholology ES-like
deposit info
lot info
Medium Medium List
Anchordepd Adherent cells
Medium and additives DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/3-4 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR (human) OK
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y.  Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.  Nat Commun  2016  7:10351  PubMed ID: 26762480



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