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APS0007 : iPS-Hep-FB/Ng/gfp-103C-1  update : 2018/07/31
CommentMouse iPS cell line derived from liver cells. Expressing GFP by CAG promoter. βgeo is knocked-in at Fbx15 locus. GFP and puromycin resistant gene can be expressed by Nanog promoter. Order Form ( C-0033, C-00034). Please see CiRA , Kyoto University if you are a researcher at a for-profit organization.
Comment from the depositor
Restriction (Category)f
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. 3) Attach TERMS and CONDITIONS to MTA (C-0034) .
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
License GFP
Basic information Depositor http://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2009
Animal mouse < Mammals
Gender Male
Age at sampling 21 weeks
Tissue liver
Classification iPS
Recombinant recombinant
Exogene pMXs, Oct3/4, Sox2, Klf4, c-Myc, GFP, βgeo gene
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10^(6) cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 4


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Reference

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User's Publication
Takagi R, Ishimaru J, Sugawara A, Toyoshima KE, Ishida K, Ogawa M, Sakakibara K, Asakawa K, Kashiwakura A, Oshima M, Minamide R, Sato A, Yoshitake T, Takeda A, Egusa H, Tsuji T.  Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model.  Sci Adv  2016  2:e1500887  PubMed ID: 27051874
Tanaka T, Yokoi T, Tamalu F, Watanabe S, Nishina S, Azuma N.  Generation of Retinal Ganglion Cells With Functional Axons From Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells.  Invest. Ophthalmol. Vis. Sci.  2016  57:3348-59  PubMed ID: 27367502
  Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells  Journal of Hard Tissue Biology  2018  27, 1-10 
Zhou Q, Ye X, Ran Q, Kitahara A, Matsumoto Y, Moriyama M, Ajioka Y, Saijo Y.  Trachea Engineering Using a Centrifugation Method and Mouse-Induced Pluripotent Stem Cells.  Tissue Eng Part C Methods  2018    PubMed ID: 30101671



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