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APS0006 : iPS-Stm-FB/gfp-99-3  update : 2018/07/31
CommentMouse iPS cell line derived from stomach cells. Expressing GFP by CAG promoter. βgeo is knocked-in at Fbx15 locus. Order Form ( C-0033, C-00034). Please see CiRA , Kyoto University if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. 3) Attach TERMS and CONDITIONS to MTA (C-0034) .
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
License GFP
Basic information Depositor http://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2009
Animal mouse < Mammals
Gender Male
Age at sampling 12 weeks
Tissue stomach
Classification iPS
Recombinant recombinant
Exogene pMXs, Oct3/4, Sox2, Klf4, c-Myc, GFP, βgeo gene
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10^(6) cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
7280  Ono H, Obana A, Usami Y, Sakai M, Nohara K, Egusa H, Sakai T.  Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells.  Biomed Res Int  2015  2015:293570  PubMed ID: 26185754



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