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APS0003 : iPS-MEF-Fb/Ng-440A-3  update : 2018/07/31
CommentMouse iPS cell line established without retrovirus vector. It has no integration of exogene. Order Form ( C-0033, C-00034). Please see CiRA , Kyoto University if you are a researcher at a for-profit organization.
Comment from the depositor
Restriction (Category)f
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. 3) Attach TERMS and CONDITIONS to MTA (C-0034) .
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
License GFP
Basic information Depositor http://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2008
Animal mouse < Mammals
Gender Female
Age at sampling embryo
Tissue embryonic fibroblast
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, c-Myc, Fbx15βgeo/βgeo, Nanog-GFP-IRES-Puro
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10^(6) cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 4


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Reference

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User's Publication
He P, Fu J, Wang DA.  Murine pluripotent stem cells derived scaffold-free cartilage grafts from a micro-cavitary hydrogel platform.  Acta Biomater  2016  35:87-97  PubMed ID: 26911880
Higuchi S, Yoshina S, Mitani S.  Inhibition of the integrin signal constitutes a mouse iPS cell niche.  Dev. Growth Differ.  2016  58:586-99  PubMed ID: 27633818
Fukunaga I, Fujimoto A, Hatakeyama K, Aoki T, Nishikawa A, Noda T, Minowa O, Kurebayashi N, Ikeda K, Kamiya K.  In Vitro Models of GJB2-Related Hearing Loss Recapitulate Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea.  Stem Cell Reports  2016  7:1023-1036  PubMed ID: 27840044
  Respective Effects of Gelatin-Coated Polydimethylsiloxane (PDMS) Substrates on Self-renewal and Cardiac Differentiation of Induced Pluripotent Stem Cells (iPSCs)  ACS Biomater. Sci. Eng  2018  4:4321-4330 



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