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APS0002 : iPS-MEF-Ng-178B-5  update : 2018/07/31
CommentMouse iPS cell line established with three factors, Oct3/4, Sox2 and Klf4, using retrovirus vector. Order Form ( C-0033, C-00034). Please see CiRA , Kyoto University if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. 3) Attach TERMS and CONDITIONS to MTA (C-0034) .
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
License GFP
Basic information Depositor
Originator Yamanaka, Shinya
Year of deposit 2008
Animal mouse < Mammals
Gender Male
Age at sampling embryo
Tissue embryonic fibroblast
Classification iPS
Recombinant recombinant
Exogene pMXs,Oct3/4, Sox2, Klf4, Nanog-GFP-IRES-Puro^r
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10^(6) cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 17

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3673  Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, Okita K, Mochiduki Y, Takizawa N, Yamanaka S.  Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.  Nat Biotechnol  2008  26(1):101-6  PubMed ID: 18059259

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User's Publication
11644  Ser-Od T, Al-Wahabi A, Inoue K, Nakajima K, Matsuzaka K, Inoue T.  Effect of EDTA-treated dentin on the differentiation of mouse iPS cells into osteogenic/odontogenic lineages in vitro and in vivo.  Dent Mater J  2019    PubMed ID: 31341145
10507  Yokoi T, Uemura T, Takamatsu K, Shintani K, Onode E, Okada M, Hidaka N, Nakamura H.  Bioabsorbable nerve conduits coated with induced pluripotent stem cell-derived neurospheres enhance axonal regeneration in sciatic nerve defects in aged mice.  J. Biomed. Mater. Res. Part B Appl. Biomater.  2018  106(5):1752-1758  PubMed ID: 28888079
10567  Hirata M, Yamaoka T.  Effect of stem cell niche elasticity/ECM protein on the self-beating cardiomyocyte differentiation of induced pluripotent stem (iPS) cells at different stages.  Acta Biomater  2018  65:44-52  PubMed ID: 29066419
10078  Otaka A, Kitagawa K, Nakaoki T, Hirata M, Fukazawa K, Ishihara K, Mahara A, Yamaoka T.  Label-Free Separation of Induced Pluripotent Stem Cells with Anti-SSEA-1 Antibody Immobilized Microfluidic Channel.  Langmuir  2017  33:1576-1582  PubMed ID: 28092949
10454  Hirata M, Yamaoka T.  Hepatocytic differentiation of iPS cells on decellularized liver tissue.  J Artif Organs  2017  20:318-325  PubMed ID: 28776092
10472  Umemura Y, Koike N, Ohashi M, Tsuchiya Y, Meng QJ, Minami Y, Hara M, Hisatomi M, Yagita K.  Involvement of posttranscriptional regulation of Clock in the emergence of circadian clock oscillation during mouse development.  Proc. Natl. Acad. Sci. U.S.A.  2017  114:E7479-E7488  PubMed ID: 28827343
9757  Higuchi S, Yoshina S, Mitani S.  Inhibition of the integrin signal constitutes a mouse iPS cell niche.  Dev. Growth Differ.  2016  58:586-99  PubMed ID: 27633818
9932  Fukunaga I, Fujimoto A, Hatakeyama K, Aoki T, Nishikawa A, Noda T, Minowa O, Kurebayashi N, Ikeda K, Kamiya K.  In Vitro Models of GJB2-Related Hearing Loss Recapitulate Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea.  Stem Cell Reports  2016  7:1023-1036  PubMed ID: 27840044
6225  Sugimoto K, Hayashi Y2.  The Characteristics of Murine iPS Cells and siRNA Transfection Under Hypoxia.  Methods Mol Biol  2016  1357:465-74  PubMed ID: 25476444
7418  Uemura T, Ikeda M, Takamatsu K, Yokoi T, Okada M, Nakamura H.  Long-term efficacy and safety outcomes of transplantation of induced pluripotent stem cell-derived neurospheres with bioabsorbable nerve conduits for peripheral nerve regeneration in mice.  Cells Tissues Organs (Print)  2015  200:78-91  PubMed ID: 25823624
6227  Yoshida K, Sato J, Takai R, Uehara O, Kurashige Y, Nishimura M, Chiba I, Saitoh M, Abiko Y  Differentiation of mouse iPS cells into ameloblast-like cells in cultures using medium conditioned by epithelial cell rests of Malassez and gelatin-coated dishes.  Med Mol Morphol  2015  48(3):138-45  PubMed ID: 25319805
6228  Ikeda M, Uemura T, Takamatsu K, Okada M, Kazuki K, Tabata Y, Ikada Y, Nakamura H.  Acceleration of peripheral nerve regeneration using nerve conduits in combination with induced pluripotent stem cell technology and a basic fibroblast growth factor drug delivery system.  J Biomed Mater Res A  2014  102(5):1370-8  PubMed ID: 23733515
6202  Sugimoto K, Yoshizawa Y, Yamada S, Igawa K, Hayashi Y, Ishizaki H.  Effects of hypoxia on pluripotency in murine iPS cells.  Microsc Res Tech  2013  76(10):1084-92  PubMed ID: 23878105
6223  Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.  Transplantation of induced pluripotent stem cell-derived neurospheres for peripheral nerve repair.  Biochem Biophys Res Commun  2012  419(1):130-5  PubMed ID: 22333572
7664  Takeuchi F, Yokota M, Yamamoto K, Nakashima E, Katsuya T, Asano H, Isono M, Nabika T, Sugiyama T, Fujioka A, Awata N, Ohnaka K, Nakatochi M, Kitajima H, Rakugi H, Nakamura J, Ohkubo T, Imai Y, Shimamoto K, Yamori Y, Yamaguchi S, Kobayashi S, Takayanagi R, Ogihara T, Kato N.  Genome-wide association study of coronary artery disease in the Japanese.  Eur. J. Hum. Genet.  2011  20:333-40  PubMed ID: 21971053
7766  Kawagoe S, Higuchi T, Meng XL, Shimada Y, Shimizu H, Hirayama R, Fukuda T, Chang H, Nakahata T, Fukada S, Ida H, Kobayashi H, Ohashi T, Eto Y.  Generation of induced pluripotent stem (iPS) cells derived from a murine model of Pompe disease and differentiation of Pompe-iPS cells into skeletal muscle cells.  Mol. Genet. Metab.  2011  104:123-8  PubMed ID: 21703893
6224  Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.  A tissue-engineered bioabsorbable nerve conduit created by three-dimensional culture of induced pluripotent stem cell-derived neurospheres.  Biomed Mater Eng  2011  21(5-6):333-9  PubMed ID: 22561252

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