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APS0001 : iPS-MEF-Ng-20D-17  update : 2018/07/31
CommentMouse induced pluripotent stem (iPS) cell line. Expressing GFP by Nanog promoter. Order Form ( C-0033, C-00034). Please see CiRA , Kyoto University if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. 3) Attach TERMS and CONDITIONS to MTA (C-0034) .
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
License GFP
Basic information Depositor
Originator Yamanaka, Shinya
Year of deposit 2007
Another name RCB2698
Animal mouse < Mammals
Gender Male
Age at sampling embryo
Tissue embryonic fibroblast
Classification iPS
Recombinant recombinant
Exogene pMXs, Oct3/4, Sox2, Klf4, c-Myc, Nanog-GFP-IRES-Puro^r
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10^(6) cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 0
User's Publication 40

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User's Publication
7028  Nair N, Calle AS, Zahra MH, Prieto-Vila M, Oo AKK, Hurley L, Vaidyanath A, Seno A, Masuda J, Iwasaki Y, Tanaka H, Kasai T, Seno M.  A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment.  Sci Rep  2017  7:6838  PubMed ID: 28754894
7041  Wu J, Platero-Luengo A, Sakurai M, Sugawara A, Gil MA, Yamauchi T, Suzuki K, Bogliotti YS, Cuello C, Morales Valencia M, Okumura D, Luo J, Vilarino M, Parrilla I, Soto DA, Martinez CA, Hishida T, Sanchez-Bautista S, Martinez-Martinez ML, Wang H, Nohalez A, Aizawa E, Martinez-Redondo P, Ocampo A, Reddy P, Roca J, Maga EA, Esteban CR, Berggren WT, Nunez Delicado E, Lajara J, Guillen I, Guillen P, Campistol JM, Martinez EA, Ross PJ, Izpisua Belmonte JC.  Interspecies Chimerism with Mammalian Pluripotent Stem Cells.  Cell  2017  168:473-486.e15  PubMed ID: 28129541
7087  Ikeda M, Imaizumi M, Yoshie S, Otsuki K, Miyake M, Hazama A, Wada I, Omori K.  Regeneration of tracheal epithelium using mouse induced pluripotent stem cells.  Acta Otolaryngol.  2016  136:373-8  PubMed ID: 26755348
7095  T Kosawada, T Koizumi, K Ugajin, Z Feng, K Goto  Novel three-dimensional micro vibration actuator for imposing dynamic stimulations to promote differentiation of iPS cells.  Microsyst Technol  2016  22:45-56 
7636  Otsu K, Kishigami R, Oikawa-Sasaki A, Fukumoto S, Yamada A, Fujiwara N, Ishizeki K, Harada H.  Differentiation of induced pluripotent stem cells into dental mesenchymal cells.  Stem Cells Dev.  2011  21:1156-64  PubMed ID: 22085204
7661  Tashiro K, Kawabata K, Omori M, Yamaguchi T, Sakurai F, Katayama K, Hayakawa T, Mizuguchi H.  Promotion of hematopoietic differentiation from mouse induced pluripotent stem cells by transient HoxB4 transduction.  Stem Cell Res  2011  8:300-11  PubMed ID: 22000550
7707  Hayashi K, Hashimoto M, Koda M, Naito AT, Murata A, Okawa A, Takahashi K, Yamazaki M.  Increase of sensitivity to mechanical stimulus after transplantation of murine induced pluripotent stem cell-derived astrocytes in a rat spinal cord injury model.  J Neurosurg Spine  2011  15:582-93  PubMed ID: 21854127
7755  Ishizuka T, Watanabe Y.  α?-Adrenoceptor stimulation enhances leukemia inhibitory factor-induced proliferation of mouse-induced pluripotent stem cells.  Eur. J. Pharmacol.  2011  668:42-56  PubMed ID: 21745467
7781  Hasegawa Y, Takahashi N, Forrest AR, Shin JW, Kinoshita Y, Suzuki H, Hayashizaki Y.  CC chemokine ligand 2 and leukemia inhibitory factor cooperatively promote pluripotency in mouse induced pluripotent cells.  Stem Cells  2011  29:1196-205  PubMed ID: 21681859
7801  Lei F, Zhao B, Haque R, Xiong X, Budgeon L, Christensen ND, Wu Y, Song J.  In vivo programming of tumor antigen-specific T lymphocytes from pluripotent stem cells to promote cancer immunosurveillance.  Cancer Res.  2011  71:4742-7  PubMed ID: 21628492
7853  Kitazawa A, Shimizu N.  Differentiation of mouse induced pluripotent stem cells into neurons using conditioned medium of dorsal root ganglia.  N Biotechnol  2011  28:326-33  PubMed ID: 21477670
7875  Kasuda S, Tatsumi K, Sakurai Y, Kato J, Taminishi S, Takeda T, Ohashi K, Okano T, Hatake K, Shima M.  Expression of coagulation factors from murine induced pluripotent stem cell-derived liver cells.  Blood Coagul. Fibrinolysis  2011  22:271-9  PubMed ID: 21415711
7909  Yukawa H, Ikeuchi M, Noguchi H, Miyamoto Y, Ikuta K, Hayashi S.  Embryonic body formation using the tapered soft stencil for cluster culture device.  Biomaterials  2011  32:3729-38  PubMed ID: 21354615
8012  Chan SS, Li HJ, Hsueh YC, Lee DS, Chen JH, Hwang SM, Chen CY, Shih E, Hsieh PC.  Fibroblast growth factor-10 promotes cardiomyocyte differentiation from embryonic and induced pluripotent stem cells.  PLoS ONE  2011  5:e14414  PubMed ID: 21203390
8063  Yamashita T, Kawai H, Tian F, Ohta Y, Abe K.  Tumorigenic development of induced pluripotent stem cells in ischemic mouse brain.  Cell Transplant  2010  20:883-91  PubMed ID: 21054935
8080  Iwamuro M, Komaki T, Kubota Y, Seita M, Kawamoto H, Yuasa T, Shahid JM, Hassan RA, Hassan WA, Nakaji S, Nishikawa Y, Kondo E, Yamamoto K, Fox IJ, Kobayashi N.  Hepatic differentiation of mouse iPS cells in vitro.  Cell Transplant  2010  19:841-7  PubMed ID: 20955659
8081  Iwamuro M, Komaki T, Kubota Y, Seita M, Kawamoto H, Yuasa T, Shahid JM, Hassan RA, Hassan WA, Nakaji S, Nishikawa Y, Kondo E, Yamamoto K, Kobayashi N.  Comparative analysis of endoderm formation efficiency between mouse ES cells and iPS cells.  Cell Transplant  2010  19:831-9  PubMed ID: 20955658
8112  Suzuki H, Shibata R, Kito T, Ishii M, Li P, Yoshikai T, Nishio N, Ito S, Numaguchi Y, Yamashita JK, Murohara T, Isobe K.  Therapeutic angiogenesis by transplantation of induced pluripotent stem cell-derived Flk-1 positive cells.  BMC Cell Biol.  2010  11:72  PubMed ID: 20860813
8216  Yukawa H, Noguchi H, Nakase I, Miyamoto Y, Oishi K, Hamajima N, Futaki S, Hayashi S.  Transduction of cell-penetrating peptides into induced pluripotent stem cells.  Cell Transplant  2010  19:901-9  PubMed ID: 20587149
8257  Tokumoto Y, Ogawa S, Nagamune T, Miyake J.  Comparison of efficiency of terminal differentiation of oligodendrocytes from induced pluripotent stem cells versus embryonic stem cells in vitro.  J. Biosci. Bioeng.  2010  109:622-8  PubMed ID: 20471604
8337  Kawai H, Yamashita T, Ohta Y, Deguchi K, Nagotani S, Zhang X, Ikeda Y, Matsuura T, Abe K.  Tridermal tumorigenesis of induced pluripotent stem cells transplanted in ischemic brain.  J. Cereb. Blood Flow Metab.  2010  30:1487-93  PubMed ID: 20216552
9503  Kitadani J, Ojima T, Iwamoto H, Tabata H, Nakamori M, Nakamura M, Hayata K, Katsuda M, Miyajima M, Yamaue H.  Cancer Vaccine Therapy Using Carcinoembryonic Antigen - expressing Dendritic Cells generated from Induced Pluripotent Stem Cells.  Sci Rep  2018  8:4569  PubMed ID: 29545628
9504  Mullen PA.  Presentation of clean livestock for slaughter.  Vet. Rec.  1997  140:634  PubMed ID: 9228699
9545  Yan Y, Yin P, Gong H, Xue Y, Zhang G, Fang B, Chen Z, Li Y, Yang C, Huang Z, Yang X, Ge J, Zou Y.  Nucleosome Assembly Protein 1-Like 1 (Nap1l1) Regulates the Proliferation of Murine Induced Pluripotent Stem Cells.  Cell. Physiol. Biochem.  2016  38:340-50  PubMed ID: 26824453
9666  Miyamoto D, Nakazawa K.  Differentiation of mouse iPS cells is dependent on embryoid body size in microwell chip culture.  J. Biosci. Bioeng.  2016  122:507-12  PubMed ID: 27090344
9710  Shirouzu Y, Yanai G, Yang KC, Sumi S.  Effects of Activin in Embryoid Bodies Expressing Fibroblast Growth Factor 5.  Cell Reprogram  2016  18:171-86  PubMed ID: 27253628
9757  Higuchi S, Yoshina S, Mitani S.  Inhibition of the integrin signal constitutes a mouse iPS cell niche.  Dev. Growth Differ.  2016  58:586-99  PubMed ID: 27633818
10340  Ogawa Y, Kaizu K, Yanagi Y, Takada S, Sakuraba H, Oishi K.  Abnormal differentiation of Sandhoff disease model mouse-derived multipotent stem cells toward a neural lineage.  PLoS ONE  2017  12:e0178978  PubMed ID: 28575132
10544  Osawa Y, Miyamoto T, Ohno S, Ohno E.  Morphological Analysis of Live Undifferentiated Cells Derived from Induced Pluripotent Stem Cells.  Stem Cells Dev.  2018  27:1-9  PubMed ID: 28978257
10974    Abstract 925: Tumorigenesis of murine iPS cell is prevented by iron depletion with downregulation of stemness markers  Cancer Research  2017  77:925-925  
11029    Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells  Journal of Hard Tissue Biology  2018  27, 1-10 
11197  Kimura Y, Kasai K, Miyata S.  Feeder-free culture for mouse induced pluripotent stem cells by using UV/ozone surface-modified substrates.  Mater Sci Eng C Mater Biol Appl  2018    PubMed ID: 30184752
11316  Chen X, Lei F, Wang L, Xiong X, Song J.  Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells.  Methods Mol. Biol.  2019    PubMed ID: 30465194
11393  Abdullah AN, Miyauchi S, Onishi A, Tanimoto K, Kato K.  Differentiation of mouse-induced pluripotent stem cells into dental epithelial-like cells in the absence of added serum.  In Vitro Cell. Dev. Biol. Anim.  2019    PubMed ID: 30659476
11441  Haque M, Lei F, Xiong X, Das JK, Ren X, Fang D, Salek-Ardakani S, Yang JM, Song J.  Stem cell-derived tissue-associated regulatory T cells suppress the activity of pathogenic cells in autoimmune diabetes.  JCI Insight  2019    PubMed ID: 30777937
11489  Kasuda S, Kudo R, Yuui K, Sakurai Y, Hatake K.  Induced Pluripotent Stem Cell-Derived Hematopoietic Embryoid Bodies Improve Mouse Status in Septic Peritonitis.  Bull. Exp. Biol. Med.  2019    PubMed ID: 30903503
4448    Stage-Dependent Differential Gene Expression Profiles of Cranial Neural Crest Cells Derived from Mouse Induced Pluripotent Stem Cells  bioRxiv  2018   
4257  Xu N, Li X, Watanabe M, Ueki H, Hu H, Li N, Araki M, Wada K, Xu A, Liu C, Nasu Y, Huang P.  Induction of cells with prostate cancer stem-like properties from mouse induced pluripotent stem cells via conditioned medium.  Am J Cancer Res  2018    PubMed ID: 30210930
4258  Hisanaga Y, Suzuki E, Aoki H, Sato M, Saito A, Saito A, Azuma T.  Effect of the combined use of enamel matrix derivative and atelocollagen sponge scaffold on osteoblastic differentiation of mouse induced pluripotent stem cells in vitro.  J. Periodont. Res.  2018    PubMed ID: 29044527
4259  Ishizuka T, Ozawa A, Katsuura M, Nomura S, Satoh Y.  Effects of muscarinic acetylcholine receptor stimulation on the differentiation of mouse induced pluripotent stem cells into neural progenitor cells.  Clin. Exp. Pharmacol. Physiol.  2018    PubMed ID: 29920752

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