Cell No. : Cell Name
AES0196 : Mvh-Venus GS
update : 2024/02/06
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| Comment | This GS cell line was derived from testis of new born transgenic mouse, Tg(Mvh-Venus)1Rbrc, carrying Venus gene inserted into the 2nd exon of Mouse Vasa Homolog (Mvh or alternatively called Ddx4) gene. Mvh gene is known to be specifically expressed in male and female germ cells. Medium and additives(excel:20KB) |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genesis 2013;51:498-505.) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Abe, Kuniya
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| Originator |
Abe, Kuniya
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| Year of deposit |
2013
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| Animal |
_mouse
< Mammals
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| Strain name |
C57BL/6J, DBA2 (mix background)
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| Gender |
Male
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| Age at sampling |
1 day
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| Tissue |
testis
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| Classification |
ES
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| Recombinant |
recombinant
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| Exogene |
Mouse Vasa homolog (Mvh or Ddx4), Venus
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| Year of origin |
2011
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Lifespan |
infinite
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| Morphology |
other
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| Cellosaurus(Expasy) |
CVCL_1T55
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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Please check [Comment].
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.2% gelatin coated dish
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Culture information
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Passage cell No |
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3 x 10 5 cells/well/6 well plate
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| SC frequency |
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Subculture : once/5 days, Medium Renewal : once/2-3days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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5 x 10 4 cells/well/6 well plate
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| Freeze medium |
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CELLBANKER 2
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Animal PCR |
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OK
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| SSLP(mouse) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
1
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