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Cell No. : Cell Name
AES0177 : Etv2-Venus-KO  update : 2024/02/06
CommentMouse embryonic stem (ES) cell line, Etv2-Venus-KO (see reference).
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Blood 2011;118:6975-6986) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) Needs MTA with RIKEN CDB regarding the purpose of the utilization of the cell line.
Remarks
approver's address
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English
Address
RIKEN
Kobe Campus
2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan
Dr.RIKEN, Center for Biosystems Dynamics Research
Fax. +81-78-306-0101
Order Form Order Form(C-0032.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishikawa, Shin-ichi
Originator Kataoka, Hiroshi
Year of deposit 2012
Animal _mouse < Mammals
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene Etv2-Venus KI
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_1Q69
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium GMEM + 1% FBS + 10% KSR + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
4598  Kataoka H, Hayashi M, Nakagawa R, Tanaka Y, Izumi N, Nishikawa S, Jakt ML, Tarui H, Nishikawa S.  Etv2/ER71 induces vascular mesoderm from Flk1+PDGFRα+ primitive mesoderm.  Blood  2011  118(26):6975-86  PubMed ID: 21911838   DOI: 10.1182/blood-2011-05-352658

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