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Cell No. : Cell Name
AES0152 : OCRG9  update : 2023/03/28
CommentMouse embryonic stem cell line derived from 129/Ola.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Development 2008;135:909-918) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2009
Animal _mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene Rex1-EGFP-IB KI vector, Oct3/4-linker-ECFP KI vectors.
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_C395
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 1
User's Publication 1


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Reference
3608  Toyooka Y, Shimosato D, Murakami K, Takahashi K, Niwa H.  Identification and characterization of subpopulations in undifferentiated ES cell culture.  Development  2008  135(5):909-18  PubMed ID: 18263842   DOI: 10.1242/dev.017400

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User's Publication
20547  Ando Y, Okeyo KO, Adachi T.  Pluripotency state of mouse ES cells determines their contribution to self-organized layer formation by mesh closure on microstructured adhesion-limiting substrates.  Biochem Biophys Res Commun  2022  590:97-102  PubMed ID: 34973536   DOI: 10.1016/j.bbrc.2021.12.066



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