Cell No. : Cell Name
AES0146 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19)
update : 2023/07/18
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| Comment | Mouse ES cell line lacking all of Dnmt1, Dnmt3a and Dnmt3b. Derived from 129Sv/Jae. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells 2006;11:805-814) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Okano, Masaki
|
| Originator |
Tsumura, Akiko & Okano, Masaki
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| Year of deposit |
2008
|
| Animal |
_mouse
< Mammals
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| Strain name |
129Sv/Jae
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
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| Classification |
ES
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| Recombinant |
recombinant
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| Exogene |
LacZ, Neomycin resistance gene, Hygromycin resistance gene, Puromycin resistance gene, IRES
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|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_1T48
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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GMEM + 15% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 4-10 split
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
|
free
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
7
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| User's Publication |
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20967
Mukamel Z, Lifshitz A, Mittnenzweig M, Chomsky E, Schwartzman O, Ben-Kiki O, Zerbib M, Tanay A.
DNA methyltransferases 3A and 3B target specific sequences during mouse gastrulation.
Nat Struct Mol Biol
2022
PubMed ID: 36510023
DOI: 10.1038/s41594-022-00885-6
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11471
Seah MKY, Wang Y, Goy PA, Loh HM, Peh WJ, Low DHP, Han BY, Wong E, Leong EL, Wolf G, Mzoughi S, Wollmann H, Macfarlan TS, Guccione E, Messerschmidt DM.
The KRAB-zinc-finger protein ZFP708 mediates epigenetic repression at RMER19B retrotransposons.
Development
2019
PubMed ID: 30846446
DOI: 10.1242/dev.170266
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11236
Chatterjee B, Lin MH, Chen CC, Peng KL, Wu MS, Tseng MC, Chen YJ, Shen CJ.
DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.
Biochim Biophys Acta Gene Regul Mech
2018
PubMed ID: 30300721
DOI: 10.1016/j.bbagrm.2018.09.009
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7029
Yagi M, Kishigami S, Tanaka A, Semi K, Mizutani E, Wakayama S, Wakayama T, Yamamoto T, Yamada Y.
Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation.
Nature
2017
548:224-227
PubMed ID: 28746308
DOI: 10.1038/nature23286
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7032
Yamazaki T, Hatano Y, Handa T, Kato S, Hoida K, Yamamura R, Fukuyama T, Uematsu T, Kobayashi N, Kimura H, Yamagata K.
Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase.
PLoS ONE
2017
12:e0177764
PubMed ID: 28542388
DOI: 10.1371/journal.pone.0177764
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10326
Miyamoto K, Uechi A, Saito K.
The zinc finger domain of RING finger protein 141 reveals a unique RING fold.
Protein Sci.
2017
26:1681-1686
PubMed ID: 28547869
DOI: 10.1002/pro.3201
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14051
Saito Y, Kunitomi A, Seki T, Tohyama S, Kusumoto D, Takei M, Kashimura S, Hashimoto H, Yozu G, Motoda C, Shimojima M, Egashira T, Oda M, Fukuda K, Yuasa S.
Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells
FEBS Lett
2017
591(18):2879-2889
PubMed ID: 28805244
DOI: 10.1002/1873-3468.12791
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