Cell No. : Cell Name
AES0137 : ZHTc6
update : 2023/07/19
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| Comment | Mouse ES cell line derived from 129/Ola. It should be cultured in the presence of tetracycline to maintain the undifferentiated state. See reference regarding more precise information. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Genet 2000;24:372) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Niwa, Hitoshi
|
| Originator |
Niwa, Hitoshi
|
| Year of deposit |
2008
|
| Animal |
_mouse
< Mammals
|
| Strain name |
129/Ola
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Recombinant |
recombinant
|
| Exogene |
Tetracycline-regulatable Oct3/4 transgene, IRESβgeopA selection marker
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_C714
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1μg/ml Tetracycline + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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0.5-1 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
|
| Feeder cells |
|
free
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
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| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
0
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| Reference |
|
4585
Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.
Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.
PLoS One
2006
1:e26.
PubMed ID: 17183653
DOI: 10.1371/journal.pone.0000026
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4578
Niwa H, Miyazaki J, Smith AG.
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.
Nat Genet
2000
24(4):372-6
PubMed ID: 10742100
DOI: 10.1038/74199
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4580
Niwa H, Burdon T, Chambers I, Smith A.
Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3.
Genes Dev
1998
12(13):2048-60
PubMed ID: 9649508
DOI: 10.1101/gad.12.13.2048
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