Cell No. : Cell Name
AES0136 : ZHBTc4
update : 2023/07/19
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| Comment | Mouse ES cell line derived from ZHTc6 and maintained by tetracycline(Tc)-regulatable Oct-3/4 transgene. Both of Oct-3/4 locus are disrupted by IRES-zeocin and IRES-BSD KO vectors. It should be cultured in the absence of tetraycline to maintain the undifferentiated state. See reference regarding more precise information. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Genet 2000;24:372) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Niwa, Hitoshi
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| Originator |
Niwa, Hitoshi
|
| Year of deposit |
2008
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| Animal |
_mouse
< Mammals
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| Strain name |
129/Ola
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Recombinant |
recombinant
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| Exogene |
Tetracycline-regulatable Oct3/4 transgene, IRESβgeopA selection marker
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_C715
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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0.5-1 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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free
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
8
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| User's Publication |
2
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| Reference |
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4588
Sharov AA, Masui S, Sharova LV, Piao Y, Aiba K, Matoba R, Xin L, Niwa H, Ko MS.
Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.
BMC Genomics
2008
9:269
PubMed ID: 18522731
DOI: 10.1186/1471-2164-9-269
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4586
Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H.
Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.
Nat Cell Biol
2007
9(6):625-35
PubMed ID: 17515932
DOI: 10.1038/ncb1589
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4584
Nakatake Y, Fukui N, Iwamatsu Y, Masui S, Takahashi K, Yagi R, Yagi K, Miyazaki J, Matoba R, Ko MS, Niwa H.
Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells.
Mol Cell Biol
2006
26(20):7772-82
PubMed ID: 16954384
DOI: 10.1128/MCB.00468-06
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4585
Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.
Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.
PLoS One
2006
1:e26.
PubMed ID: 17183653
DOI: 10.1371/journal.pone.0000026
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4582
Miyagi S, Saito T, Mizutani K, Masuyama N, Gotoh Y, Iwama A, Nakauchi H, Masui S, Niwa H, Nishimoto M, Muramatsu M, Okuda A.
The Sox-2 regulatory regions display their activities in two distinct types of multipotent stem cells.
Mol Cell Biol
2004
24(10):4207-20
PubMed ID: 15121842
DOI: 10.1128/mcb.24.10.4207-4220.2004
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4579
Niwa H, Masui S, Chambers I, Smith AG, Miyazaki J.
Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells.
Mol Cell Biol
2002
22(5):1526-36
PubMed ID: 11839818
DOI: 10.1128/mcb.22.5.1526-1536.2002
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4578
Niwa H, Miyazaki J, Smith AG.
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.
Nat Genet
2000
24(4):372-6
PubMed ID: 10742100
DOI: 10.1038/74199
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4580
Niwa H, Burdon T, Chambers I, Smith A.
Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3.
Genes Dev
1998
12(13):2048-60
PubMed ID: 9649508
DOI: 10.1101/gad.12.13.2048
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| User's Publication |
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13142
Oh S, Boo K, Kim J, Baek SA, Jeon Y, You J, Lee H, Choi HJ, Park D, Lee JM, Baek SH.
The chromatin-binding protein PHF6 functions as an E3 ubiquitin ligase of H2BK120 via H2BK12Ac recognition for activation of trophectodermal genes.
Nucleic Acids Res
2020
PubMed ID: 32735658
DOI: 10.1093/nar/gkaa626
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15215
Boo K, Bhin J, Jeon Y, Kim J, Shin HJ, Park JE, Kim K, Kim CR, Jang H, Kim IH, Kim VN, Hwang D, Lee H, Baek SH.
Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells
Nat Commun
2015
6:6810
PubMed ID: 25857206
DOI: 10.1038/ncomms7810
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