Cell No. : Cell Name
AES0135 : E14tg2a
update : 2024/08/14
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| Comment | Mouse embryonic stem (ES) cell line derived from 129/Ola. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nature 1987;326:292) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Niwa, Hitoshi
|
| Originator |
Martin Hooper
|
| Year of deposit |
2008
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| Animal |
_mouse
< Mammals
|
| Strain name |
129/Ola
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
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| Classification |
ES
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Lifespan |
infinite
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| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_9108
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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0.5-1 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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free
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Chromosome mode |
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40(47), 41(2), 42(1)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
18
|
| User's Publication |
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23226
Jia X, Isomura A, Kageyama R.
Cdh2, a downstream target of Hes7, regulates somitogenesis by supporting FGF signalling.
Development
2025
152(17)
PubMed ID: 40951951
DOI: 10.1242/dev.204743
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21640
Lin YC, Wuputra K, Kato K, Ku CC, Saito S, Noguchi M, Nakamura Y, Hsiao M, Lin CS, Wu DC, Kawaguchi A, Yu HS, Yokoyama KK.
Di-n-butyl phthalate promotes the neural differentiation of mouse embryonic stem cells through neurogenic differentiation 1.
Environ Pollut
2024
123722
PubMed ID: 38460589
DOI: 10.1016/j.envpol.2024.123722
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22246
Ohishi H, Shinkai S, Owada H, Fujii T, Hosoda K, Onami S, Yamamoto T, Ohkawa Y, Ochiai H.
Transcription-coupled changes in genomic region proximities during transcriptional bursting.
Sci Adv
2024
10(49):eadn0020
PubMed ID: 39642226
DOI: 10.1126/sciadv.adn0020
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21396
Okamoto K, Fujita H, Okada Y, Shinkai S, Onami S, Abe K, Fujimoto K, Sasaki K, Shioi G, Watanabe TM.
Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance.
EMBO J
2023
e112305
PubMed ID: 37609947
DOI: 10.15252/embj.2022112305
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17678
Sanaki-Matsumiya M, Kageyama R.
Time-Lapse Bioluminescence Imaging of Hes7 Expression In Vitro and Ex Vivo
Methods Mol Biol
2022
2525:321-332.
PubMed ID: 35836080
DOI: 10.1007/978-1-0716-2473-9_25
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13386
Kaitsuka T, Tomizawa K.
Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells Through Protein Transduction of Transcription Factors.
Methods Mol Biol
2021
PubMed ID: 33336272
DOI: 10.1007/978-1-0716-0943-9_7
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20172
Ando Y, Okeyo KO, Sunaga J, Adachi T.
Edge-localized alteration in pluripotency state of mouse ES cells forming topography-confined layers on designed mesh substrates.
Stem Cell Res
2021
53:102352
PubMed ID: 33901814
DOI: 10.1016/j.scr.2021.102352
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20195
Okamoto K, Watanabe TM, Horie M, Nishiyama M, Harada Y, Fujita H.
Pressure-induced changes on the morphology and gene expression in mammalian cells.
Biol Open
2021
PubMed ID: 34128043
DOI: 10.1242/bio.058544
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20419
Kaneshiro J, Shioi G, Okamoto K, Onami S, Watanabe TM.
Improvement in image quality via the pseudo confocal effect in multidirectional digital scanned laser light-sheet microscopy.
Opt Express
2021
29(15):24278-24288
PubMed ID: 34614676
DOI: 10.1364/OE.423783
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6254
Maiko Yamamoto, Yoshiaki Suwa, Kohta Sugiyama, Naoki Okashita, Masanori Kawaguchi, Naoki Tani, Kazumi Matsubara, Akira Nakamura, Yoshiyuki Seki
The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency
J Cell Sci
2020
133(15):jcs240176
PubMed ID: 32661086
DOI: 10.1242/jcs.240176
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11578
Panina Y, Germond A, David BG, Watanabe TM.
Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay.
BMC Bioinformatics
2019
PubMed ID: 31146686
DOI: 10.1186/s12859-019-2911-5
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11703
Brit Gracy David, Hideaki Fujita, Kyota Yasuda, Kazuko Okamoto, Yulia Panina, Junya Ichinose, Osamu Sato, Masanobu Horie, Taro Ichimura, Yasushi Okada, Tomonobu M Watanabe
Linking substrate and nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonic stem cells
Stem Cell Research
2019
PubMed ID: 31715427
DOI: 10.1016/j.scr.2019.101614
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12412
Ando Y, Okeyo KO, Adachi T.
Modulation of adhesion microenvironment using mesh substrates triggers self-organization and primordial germ cell-like differentiation in mouse ES cells
PL Bioeng
2019
3(1):016102
PubMed ID: 31069335
DOI: 10.1063/1.5072761
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10741
Matsumiya M, Tomita T, Yoshioka-Kobayashi K, Isomura A, Kageyama R.
ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock.
Development
2018
145:
PubMed ID: 29437832
DOI: 10.1242/dev.156836
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10792
Kobayashi N, Kawasaki-Nishi S, Otsuka M, Hisano Y, Yamaguchi A, Nishi T.
MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells.
Sci Rep
2018
8:4969
PubMed ID: 29563527
DOI: 10.1038/s41598-018-23300-x
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11162
Okamoto K, Germond A, Fujita H, Furusawa C, Okada Y, Watanabe TM.
Single cell analysis reveals a biophysical aspect of collective cell-state transition in embryonic stem cell differentiation.
Sci Rep
2018
8(1):11965
PubMed ID: 30097661
DOI: 10.1038/s41598-018-30461-2
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10077
Ashida Y, Nakajima-Koyama M, Hirota A, Yamamoto T, Nishida E.
Activin A in combination with ERK1/2 MAPK pathway inhibition sustains propagation of mouse embryonic stem cells.
Genes Cells
2017
22:189-202
PubMed ID: 28097777
DOI: 10.1111/gtc.12467
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9720
Hidema S, Fukuda T, Date S, Tokitake Y, Matsui Y, Sasaki H, Nishimori K.
Transgenic expression of Telomerase reverse transcriptase (Tert) improves cell proliferation of primary cells and enhances reprogramming efficiency into the induced pluripotent stem cell.
Biosci. Biotechnol. Biochem.
2016
80:1925-33
PubMed ID: 27297181
DOI: 10.1080/09168451.2016.1191330
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