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Cell No. : Cell Name
AES0126 : Nanog^(βgeo/Hyg)  update : 2022/12/21
CommentA subline of mouse embryonic stem cell (RF8 derived from 129 SV Jae strain mouse) lacking Nanog expression. Nanog genes were replaced with βgeo gene in one allele and hygromycin resistant gene in another allele. Feeder-free cell line.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA.
Remarks
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor https://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2007
Another name RCB2697
Animal _mouse < Mammals
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene βgeo gene, hygr gene
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_T796
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3669  Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S  The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells.  Cell  2003  113(5):631-42  PubMed ID: 12787504   DOI: 10.1016/s0092-8674(03)00393-3

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