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AES0014 : TT2  update : 2018/07/06
CommentMouse ES cell line (B6 X CBA). This cell line can be used only for in vitro culture experiment.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Anal Biochem 1993;214:70-76). 2) Remarks:Germline-differentiating potency is not guaranteed. The TT2 cells with high germline-differentiating potency will be distributed by Laboratory for Animal Resources and Genetic Engineering (LARGE:mutant@cdb.riken.jp), RIKEN Center for Biosystems Dynamics Research.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Aizawa, Shinichi
Originator Tokunaga, T. & Aizawa, S.
Year of deposit 2005
Another name RCB2077
Animal mouse < Mammals
Strain name C57BL/6 x CBA
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, 1mM Sodium Pyruvate) + 20% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10^(5) cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 4


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Reference
3268  Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M, Tsukada T, Saga Y, Takeda N, Ikawa Y, Aizawa S  A novel ES cell line, TT2, with high germline-differentiating potency.  Anal Biochem  1993  214:70-6  PubMed ID: 8250257

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User's Publication
11168  Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S.  Zfat expression in ZsGreen reporter gene knock?in mice: Implications for a novel function of Zfat in definitive erythropoiesis.  Int. J. Mol. Med.  2018  42(5):2595-2603  PubMed ID: 30106088
10256  Yang Y, Liu B, Xu J, Wang J, Wu J, Shi C, Xu Y, Dong J, Wang C, Lai W, Zhu J, Xiong L, Zhu D, Li X, Yang W, Yamauchi T, Sugawara A, Li Z, Sun F, Li X, Li C, He A, Du Y, Wang T, Zhao C, Li H, Chi X, Zhang H, Liu Y, Li C, Duo S, Yin M, Shen H, Belmonte JCI, Deng H.  Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency.  Cell  2017  169:243-257.e25  PubMed ID: 28388409
9579  Kanegi R, Hatoya S, Tsujimoto Y, Takenaka S, Nishimura T, Wijewardana V, Sugiura K, Takahashi M, Kawate N, Tamada H, Inaba T.  Production of feline leukemia inhibitory factor with biological activity in Escherichia coli.  Theriogenology  2016  86:604-11  PubMed ID: 27020881
5313  Ishisaka A, Mukai R, Terao J, Shibata N, Kawai Y.  Specific localization of quercetin-3-O-glucuronide in human brain.  Arch Biochem Biophys  2014  557:11-7  PubMed ID: 24893148



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