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AES0003 : B6G-2
update : 2018/07/06
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| Comment | Embryonic stem (ES) cell line derived from C57BL/6 mouse. Expressing GFP. |
| Comment from the depositor | |
| Terms and conditions | The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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License |
GFP
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Basic information
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Depositor |
Sugiyama, Fumihiro
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| Originator |
Sugiyama, Fumihiro
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| Year of deposit |
2005
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| Another name |
RCB2001
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| Animal |
mouse
< Mammals
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| Strain name |
C57BL/6
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Classification |
ES
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| Recombinant |
recombinant
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| Exogene |
EGFP
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Lifespan |
infinite
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| Morphology |
ES-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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DMEM (high glucose, 1mM Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% trypsin + 0.02% EDTA
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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2 x 10^(5) cells/60 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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MEF (BALB/c)
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10^(5) cells/60 mm dish
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
5
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| Reference |
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3092
Shimizukawa, Rie, Sakata, Aya, Hirose, Michiko, Takahashi, Akio, Iseki, Hiroyoshi, Liu, Ying, Kunita, Satoshi, Sugiyama, Fumihiro, Yagami, Ken-ichi
Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously.
Genesis
2005
42:47-52
PubMed ID: 15830376
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| User's Publication |
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7377
Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Matsumoto T, Nakata K, Mogi M.
Interleukin-1β-induced autophagy-related gene 5 regulates proliferation of embryonic stem cell-derived odontoblastic cells.
PLoS ONE
2015
10:e0124542
PubMed ID: 25894570
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8095
Ishikawa K, Toyama-Sorimachi N, Nakada K, Morimoto M, Imanishi H, Yoshizaki M, Sasawatari S, Niikura M, Takenaga K, Yonekawa H, Hayashi J.
The innate immune system in host mice targets cells with allogenic mitochondrial DNA.
J. Exp. Med.
2010
207:2297-305
PubMed ID: 20937705
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8243
Hossain MM, Shimizu E, Saito M, Rao SR, Yamaguchi Y, Tamiya E.
Non-invasive characterization of mouse embryonic stem cell derived cardiomyocytes based on the intensity variation in digital beating video.
Analyst
2010
135:1624-30
PubMed ID: 20517541
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8409
Hayashi Y, Furue MK, Tanaka S, Hirose M, Wakisaka N, Danno H, Ohnuma K, Oeda S, Aihara Y, Shiota K, Ogura A, Ishiura S, Asashima M.
BMP4 induction of trophoblast from mouse embryonic stem cells in defined culture conditions on laminin.
In Vitro Cell. Dev. Biol. Anim.
2009
46:416-30
PubMed ID: 20033790
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6195
Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K, Mogi M.
IL-1β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway.
Exp Cell Res
2014
328(1):69-86
PubMed ID: 24851717
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