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AES0003 : B6G-2  update : 2018/07/06
CommentEmbryonic stem (ES) cell line derived from C57BL/6 mouse. Expressing GFP.
Comment from the depositor
Terms and conditionsThe RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
License GFP
Basic information Depositor Sugiyama, Fumihiro
Originator Sugiyama, Fumihiro
Year of deposit 2005
Another name RCB2001
Animal mouse < Mammals
Strain name C57BL/6
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene EGFP
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, 1mM Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF (BALB/c)
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 5


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Reference
3092  Shimizukawa, Rie, Sakata, Aya, Hirose, Michiko, Takahashi, Akio, Iseki, Hiroyoshi, Liu, Ying, Kunita, Satoshi, Sugiyama, Fumihiro, Yagami, Ken-ichi  Establishment of a new embryonic stem cell line derived from C57BL/6 mouse expressing EGFP ubiquitously.  Genesis  2005  42:47-52  PubMed ID: 15830376

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User's Publication
7377  Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Matsumoto T, Nakata K, Mogi M.  Interleukin-1β-induced autophagy-related gene 5 regulates proliferation of embryonic stem cell-derived odontoblastic cells.  PLoS ONE  2015  10:e0124542  PubMed ID: 25894570
8095  Ishikawa K, Toyama-Sorimachi N, Nakada K, Morimoto M, Imanishi H, Yoshizaki M, Sasawatari S, Niikura M, Takenaga K, Yonekawa H, Hayashi J.  The innate immune system in host mice targets cells with allogenic mitochondrial DNA.  J. Exp. Med.  2010  207:2297-305  PubMed ID: 20937705
8243  Hossain MM, Shimizu E, Saito M, Rao SR, Yamaguchi Y, Tamiya E.  Non-invasive characterization of mouse embryonic stem cell derived cardiomyocytes based on the intensity variation in digital beating video.  Analyst  2010  135:1624-30  PubMed ID: 20517541
8409  Hayashi Y, Furue MK, Tanaka S, Hirose M, Wakisaka N, Danno H, Ohnuma K, Oeda S, Aihara Y, Shiota K, Ogura A, Ishiura S, Asashima M.  BMP4 induction of trophoblast from mouse embryonic stem cells in defined culture conditions on laminin.  In Vitro Cell. Dev. Biol. Anim.  2009  46:416-30  PubMed ID: 20033790
6195  Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K, Mogi M.  IL-1β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway.  Exp Cell Res  2014  328(1):69-86  PubMed ID: 24851717



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